应用原位分子杂交及免疫组化方法，使用地高辛标记ＨＣＶ５′非编码区探针及抗ＨＣＶＮＳ３区Ｃ３３ｃ单克隆抗体，对３５例人原发性肝内胆管细胞癌（ＰＩＣ）和癌旁肝组织内ＨＣＶＲＮＡ及其ＮＳ抗原进行检测，结果发现ＨＣＶＲＮＡ在ＰＩＣ中的阳性率为８３％，ＨＣＶＲＮＡ定位于癌细胞胞浆中。个别病例并在淋巴细胞、肝窦内皮细胞和枯否氏细胞中发现阳性信号。ＨＣＶＮＳ３区Ｃ３３ｃ抗原在ＰＩＣ的阳性率为８９％，阳性反应主要位于癌细胞胞浆内或肝细胞胞浆内。阳性细胞在癌组织中以灶性分布为主，在癌旁肝以弥漫分布为主。ＨＢｘＡｇ在本组病例的阳性率为７７％。结果提示：在肝内胆管细胞癌的发生中，除ＨＢＶ以外．ＨＣＶ感染与其发生也有密切关系，是其致病因素之一。

CV RNA and its NS3 region C33c antigen were detected by in situ hybridization withdigoxgenin-11-dUTP labeled HCV 5'non coding region cDNA probe and immunohistochemicalmethods with monoclonal antibody against recombinant HCV C33c protein in 35 cases of primaryintrahepatcr-cholangiocarcinoma(PIC) tissues.The positive rate of HCV RNA in PIC was 83%(29/35) and the HCV RNA was localized in the cytoplasm and/or nucleus of cancerous cell andhepatocytes.In one case,HCV RNA was also detected in the nuclus of lymphocyte and sinusoidalcell/or kupffer′S cells.The positive rate of HCV NS3 region C33c antigen was 89%(31/35)inPIC,the positive reaction was found in the cytoplasm of cancerous cell and hepatocytes,the posi-tive cells distributed in local and diffusely.HBxAg was also detected in 77%(37/45)of the pa-tients studied.Our results suggest that HCV infection has closely relationship with the develop-ment of PIC except HBV infection.HCV infection may play an important role in the carcinogene-sis of PIC.