WU Lei and SHU Jia-Hong. Biological properties of a new strain of recombinant vaccinia virus expressing rabies virus glycopro- tein[J]. Virologica Sinica, 1996, 11(1).
Citation: WU Lei, SHU Jia-Hong. Biological properties of a new strain of recombinant vaccinia virus expressing rabies virus glycopro- tein .VIROLOGICA SINICA, 1996, 11(1) : 34.

一株新的狂犬病重组痘苗病毒某些生物学性质的研究

  • 对表达狂犬病毒糖蛋白的重组痘苗病毒VVM11KRG株生物学性质进行了研究,该重组病毒的特点是:(1)糖蛋白基因插入到痘苗病毒天坛株基因组HindⅢM片段中;(2)启动子为痘苗病毒天坛株P11晚期启动子;(3)不含外源lac基因。该重组病毒在CV-1细胞和鸡胚细胞上繁殖滴渡略高于另一株重组痘苗病毒VVTK11KRG,在鸡胚细胞中繁殖滴度第三天达到最高,在CV-1细胞中第二天达到最高。温度稳定性与天坛株相比没有明显改变。重组病毒在家兔皮内的毒力比亲本株(天坛株)低。间接免疫荧光和Westernblot都证明了狂犬病毒糖蛋白有良好的表达。通过Southernblot证实糖蛋白基因准确地插入到HindⅢM片段中。重组痘苗病毒启动子与部分糖蛋白基因克隆到pGEM3zf(-)质粒上,对该段DNA序列分析表明不会产生移码和融合蛋白,从而为该重组病毒的使用提供了明确的基因背景资料。

Biological properties of a new strain of recombinant vaccinia virus expressing rabies virus glycopro- tein

  • the recombinant vaccinia virus(VVM11KRG strain)contains rabies virus glycoprotein gene(RVGG) which was inserted into the Hind ⅢM fragment of vaccinia virus(V.V)genome.TheRVGG was promoted by pll promoter of V.V.The VVM11KRG does not contain lac gene.Thestudy showed that its titer is higher than that of another recombinant vaccinia virus(VVTK11KRG strain)in chicken embryo cell and CV-1 cell.Its titer reaches the highest point inchicken embryo cell in the third day and in CV-1 cell in the second day after inoculation Its sta-bility is no difference comparing with parent strain at different temperatures.The virulence ofVVM11KRG is lower than that of vaccinia virus.Western blot and IFA proved that rabies virusG protein was expressed.Southern blot showed that the RVGG was inserted into Hind Ⅲ Mfragment of vaccinia virus.The fragment between glycoprotein gene and promoter was cloned toplasmid pGEM3 zf(-).The DNA was sequenced by Pharmacia LKB DNA sequencer.Thus weobtained a clear DNA reference data for the usage of

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    Biological properties of a new strain of recombinant vaccinia virus expressing rabies virus glycopro- tein

    • 1. Wuhan Institute of Biologial Products

    Abstract: the recombinant vaccinia virus(VVM11KRG strain)contains rabies virus glycoprotein gene(RVGG) which was inserted into the Hind ⅢM fragment of vaccinia virus(V.V)genome.TheRVGG was promoted by pll promoter of V.V.The VVM11KRG does not contain lac gene.Thestudy showed that its titer is higher than that of another recombinant vaccinia virus(VVTK11KRG strain)in chicken embryo cell and CV-1 cell.Its titer reaches the highest point inchicken embryo cell in the third day and in CV-1 cell in the second day after inoculation Its sta-bility is no difference comparing with parent strain at different temperatures.The virulence ofVVM11KRG is lower than that of vaccinia virus.Western blot and IFA proved that rabies virusG protein was expressed.Southern blot showed that the RVGG was inserted into Hind Ⅲ Mfragment of vaccinia virus.The fragment between glycoprotein gene and promoter was cloned toplasmid pGEM3 zf(-).The DNA was sequenced by Pharmacia LKB DNA sequencer.Thus weobtained a clear DNA reference data for the usage of

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