应用放射性同位素￣（４５）Ｃａ￣（２＋）示踪技术及光敏生物素标记ｃＤＮＡ探针杂交方法，观察了维拉帕米、黄芪、地塞米松等药物对感染柯萨奇Ｂ＿３病毒（ＣＶＢ＿３）的大鼠培养心肌细胞Ｃａ￣（２＋）内流及细胞中ＣＶＢ＿３－ＲＮＡ复制的作用。结果发现：在病毒感染４８ｈ，上述三药均可显著减少感染细胞及正常对照的心肌Ｃａ￣（２＋）内流（Ｐ＜０．０１和Ｐ＜０．０５）；若在病毒感染后即加入上述药物，经４８ｈ培养后，黄芪组细胞中的ＣＶＢ＿３－ＲＮＡ含量显著少于病毒对照组（Ｐ＜０．００１），维拉帕米则显著增加（Ｐ＜０．０１）．而地塞米松对其无影响。提示黄芪与地塞米松具有一种与维拉帕米相似的减少病毒感染心肌Ｃａ￣（２＋）内流的作用，有可能减轻感染细胞的继发性Ｃａ￣（２＋）损伤；但三种药物对感染细胞中病毒核酸的复制有不同作用，可作为临床治疗病毒性心肌炎时的参考。

he effect of Verapamil(Ver),Astragalus membranaceus(AM),Dexamethasone(Dex)on Ca￣(2+) influx across the myocardial plasma membrane and coxsackie virus B3(CVB3)-RNA replica-tion in cultured neonatal rat heart cells infected with CVB3 was investigated.It was found thatthe Ca￣(2+) influx could be decreased significantly (p< 0.01)by Ver,AM,Dex after infection for48h.In addtion,the aniounts of CVB3-RNA in cultured heart cells infected with CVB3、and treat-ed with AM for 48h simultaneously were significantly decreased as compared with the heart cellsinfected with CVB3 only(p< 0.001) and it was significantly higher in myocytes infected withCVB3 and treated with Ver than in myocytes only infected with CVB,(p< 0.01).These resultsdemonstrated that the above three drugs could exert the effect of decreasing the secondary dam-ages caused by Ca￣(2+)influx,but their effects on replication of CVB3-RNA in myocardium werequite different:AM inhibited replicantion of CVB3+RNA.