采用黄瓜花叶病毒（ＣＭＶ）亚组Ⅰ株系Ｆｎｙ－ＣＭＶＲＮＡ＿２的１２０９～１６２６核苷酸片段和亚组Ⅱ株系Ｌｓ－ＣＭＶＲＮＡ＿２的２００２～２４３３核苷酸片段的ｃＤＮＡ克隆，体外转录，同时掺入￣（３２）Ｐ获得负链ＲＮＡ探针，与纯化的番茄和甜椒上的ＣＭＶ中国分离物的ＲＮＡ杂交，结果表明：ＣＭＶ番茄和甜椒中国分离物与Ｆｎｙ－ＣＭＶ的核苷酸有高度同源性，隶属于Ｆｎｙ－ＣＭＶ为代表的亚组Ⅰ株系。并利用Ｋ－ＣＭＶ株系（亚组Ⅰ，源于中国）的ＲＮＡ＿２全长ｃＤＮＡ克隆的两个ＥｃｏＲＩ位点间的核苷酸序列（１６５７～２１２５ｎｔ）作探针，与上述两种ＣＭＶ中国分离物的ＲＮＡ杂交，进一步比较分析了这两个分离物和Ｋ－ＣＭＶ株系的关系。讨论了核酸酶保护法在ＣＭＶ株系鉴定中的作用。

he cDNA sequences spanning nt 1209 to nt 1626 of Fny-CMV RNA2(a subgroupⅠstrain)and nt 2002 to nt 2433 of Ls-CMV RNA2(a subgroup Ⅱ strain)were used for probe preparation.The 32P-labeled negative strand RNAs prepared by transcription in vitro were hybridized with pu-rified RNAs of two Chinese CMV isolates from pepper and tomato,respectively.The resultshows that the two Chineses isolates share high identities of sequence to the Fny-CMV,thereforethe isolates belong to the CMV subgroupⅠ strain.A transcription probe complementary to thefragment flanking nt 1657 to nt 2125 of K-CMV RNA2(a subgroupⅠstrain originated from Chi-na) was hybridized with the purified RNAs of the two Chinese CMV isolates as well.The rela-tionship between the K-CMV strain and the two Chinese isolates was compared. The applicationof ribonuclease protection assay was discussed in the identification of CMV strains.