AA-ELISA for Detection of Barley Resistance to BYDV

LIU Chang-Hong

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Volume 11 Issue 1

February 1996

LIU Chang-Hong. AA-ELISA for Detection of Barley Resistance to BYDV[J]. Virologica Sinica, 1996, 11(1).

Citation: LIU Chang-Hong. AA-ELISA for Detection of Barley Resistance to BYDV .VIROLOGICA SINICA, 1996, 11(1) : 84.

检测大麦抗BYDV的蚜虫获毒的酶联免疫吸附法

刘常宏

1.
陕西省植物保护研究所

摘要

检测大麦抗ＢＹＤＶ的蚜虫获毒的酶联免疫吸附法刘常宏（陕西省植物保护研究所，陕西杨陵７１２１００）Ｓ．Ｈａｂｅｒ（ＡｇｒｉｃｕｌｔｕｒｅＣａｎａｄａ，ＷｉｎｎｉｐｅｇＲｅｓｅａｒｃｈＳｔａｔｉｏｎ，Ｃａｎａｄａ）关键词酶联免疫吸附法，大麦，大麦黄矮病毒...
- 酶联免疫吸附法大麦大麦黄矮病毒

AA-ELISA for Detection of Barley Resistance to BYDV

LIU Chang-Hong

1.
Shanxi Institute of Plant protection-Yangling，Shanxi 712100

Abstract

wo viruliferous Rhopalosiphum padi(BYDV)can be detected by indirect enzyme-linkedimmunosorbent assaay(ELISA).Based on that result,a Aphid Acquisition-Enzyme-linked im-munosorbent assay(AA-ELISA)has been established for identification of barley resistance toBYDV.The OD value of AA-ELSA showed that the barley variety 877-16 carrying YD2 genewas significantly different from that of susceptible variety Manley.No significant difference wasfound between 877-16 and Manley when they were detected directly by ELISA at the same leave.
- ELISA Barley BYDV

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

AA-ELISA for Detection of Barley Resistance to BYDV

LIU Chang-Hong

1. Shanxi Institute of Plant protection-Yangling，Shanxi 712100

Abstract: wo viruliferous Rhopalosiphum padi(BYDV)can be detected by indirect enzyme-linkedimmunosorbent assaay(ELISA).Based on that result,a Aphid Acquisition-Enzyme-linked im-munosorbent assay(AA-ELISA)has been established for identification of barley resistance toBYDV.The OD value of AA-ELSA showed that the barley variety 877-16 carrying YD2 genewas significantly different from that of susceptible variety Manley.No significant difference wasfound between 877-16 and Manley when they were detected directly by ELISA at the same leave.