DONG Jiang-Li, HA Shi-A-Gu-La and ZHANG He-Ling. The cDNA cloning and nucleotide sequence analysis of potato leaf roll virus intergenic region[J]. Virologica Sinica, 1996, 11(2).
Citation: DONG Jiang-Li, HA Shi-A-Gu-La, ZHANG He-Ling. The cDNA cloning and nucleotide sequence analysis of potato leaf roll virus intergenic region .VIROLOGICA SINICA, 1996, 11(2) : 144.

马铃薯卷叶病毒基因间隔区的克隆及序列分析

  • 根据已报道的马铃薯卷叶病毒基因组序列.设计合成一对特异性引物,以马铃薯卷叶病毒中国分离株(PLRV-Ch)的RNA为模板,反转录合成cDNA第一条链,经PCR扩增后克隆于pUC19质粒中,进一步用PCR鉴定、限制酶切分析和序列分析,结果表明:PLRV-Ch基因间隔区由197个核苷酸组成,与国外报道的荷兰PLRV-N加拿大PLRV-C,澳大利亚PLRV-A,苏格兰PLRV-S各株系核苷酸序列具有很高的同源性,同源率依次为99%、98%、93%、98%。

The cDNA cloning and nucleotide sequence analysis of potato leaf roll virus intergenic region

  • Two specific primers were designed and synthesized according to the genomic sequence ofPLRV reported in literature,The first strand of cDNA was synthesized by reverse-transcriptionusing RNA of PLRV Chinese isolate(PLRV-Ch)as a template,followed by PCR amplification.The synthesized cDNA was cloned into plasmid pUC19 in DH5α.The cDNA clone was further i-dentified by PCR,restriction enzymes analysis and nucleotide sequence analysis.Tests show thatthe intergenic region of PLRV-Ch isolate consists of 197 nucleotides,and there is a high homologyin nucleotide sequence in comparision with PLRV Netherland isolate(PLRV-N),PLRV Canadianisolate(PLRV-C),PLRV Austrilian isolate (PLRV-A)and PLRV Scottish (PLRV-S)The rate ofnucleotide sequence homobgy is 99%,98%, 93%and 98%,respectively.

  • 加载中
  • 加载中

Article Metrics

Article views(3641) PDF downloads(759) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    The cDNA cloning and nucleotide sequence analysis of potato leaf roll virus intergenic region

    • 1. Dep. of Biology, Inner Mongonia University

    Abstract: Two specific primers were designed and synthesized according to the genomic sequence ofPLRV reported in literature,The first strand of cDNA was synthesized by reverse-transcriptionusing RNA of PLRV Chinese isolate(PLRV-Ch)as a template,followed by PCR amplification.The synthesized cDNA was cloned into plasmid pUC19 in DH5α.The cDNA clone was further i-dentified by PCR,restriction enzymes analysis and nucleotide sequence analysis.Tests show thatthe intergenic region of PLRV-Ch isolate consists of 197 nucleotides,and there is a high homologyin nucleotide sequence in comparision with PLRV Netherland isolate(PLRV-N),PLRV Canadianisolate(PLRV-C),PLRV Austrilian isolate (PLRV-A)and PLRV Scottish (PLRV-S)The rate ofnucleotide sequence homobgy is 99%,98%, 93%and 98%,respectively.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return