Rapid Purification and in vitro Translation of Heliothis armigera
Nuclear Polyhedrosis Virus mRNA

WU Jin-Lu; LIU Run-Zhong; ZHANG You-Qing

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April 1996

WU Jin-Lu, LIU Run-Zhong and ZHANG You-Qing. Rapid Purification and in vitro Translation of Heliothis armigeraNuclear Polyhedrosis Virus mRNA[J]. Virologica Sinica, 1996, 11(2).

Citation: WU Jin-Lu, LIU Run-Zhong, ZHANG You-Qing. Rapid Purification and in vitro Translation of Heliothis armigera Nuclear Polyhedrosis Virus mRNA .VIROLOGICA SINICA, 1996, 11(2) : 183.

棉铃虫核型多角体病毒mRNA的快速制备与体外翻译

1.
中国科学院武汉病毒研究所福建省水产研究所病防室

摘要

棉铃虫核型多角体病毒ｍＲＮＡ的快速制备与体外翻译吴金炉，刘润忠，张友清（中国科学院武汉病毒研究所，武汉４３００７１）关键词棉铃虫核型多角体病毒，ｍＲＮＡ，快速制备，体外翻译，ＥＬＩＳＡ在昆虫杆状病毒ｍＲＮＡ的结构与功能研究方面，主要是以ＡｃＮＰＶ为研...
- 棉铃虫核型多角体病毒 mRNA 快速制备体外翻译 ELISA

Rapid Purification and in vitro Translation of Heliothis armigera Nuclear Polyhedrosis Virus mRNA

1.
Wuhan Institute of Virology．Academia Sinica，Wuhan 430071

Abstract

Total RNAs were isolated from Heliothis armigera larvae infected with Heliothis armigeraNuclear Polyhedrosis Virus(HaNPV) late after infection(60 h postinfection),800 μg total RNAswere obtained from 1g larvae by improved granidinium isothiocgarate method.The whole viralDNA was purified from HaNPV polyhedra.The DNA was immobilized on nitrocellulose filtersand used to select viral specific mRNAs from total RNA.The specific mRNAs were translated invitro in the presence of L-(35S)methionine by using a rabbit reticulocyte lysate system and ana-lyzed on sodium dodecyl sulfate-polyacrylamide gel.At least twenty proteins were obtained.Theviral structural proteins were among the translation products which were testified with the experi-ment of ELISA.
- HaNPV mRNA Rapid purification in vitro translation ELISA

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rapid Purification and in vitro Translation of Heliothis armigera Nuclear Polyhedrosis Virus mRNA

1. Wuhan Institute of Virology．Academia Sinica，Wuhan 430071

Abstract: Total RNAs were isolated from Heliothis armigera larvae infected with Heliothis armigeraNuclear Polyhedrosis Virus(HaNPV) late after infection(60 h postinfection),800 μg total RNAswere obtained from 1g larvae by improved granidinium isothiocgarate method.The whole viralDNA was purified from HaNPV polyhedra.The DNA was immobilized on nitrocellulose filtersand used to select viral specific mRNAs from total RNA.The specific mRNAs were translated invitro in the presence of L-(35S)methionine by using a rabbit reticulocyte lysate system and ana-lyzed on sodium dodecyl sulfate-polyacrylamide gel.At least twenty proteins were obtained.Theviral structural proteins were among the translation products which were testified with the experi-ment of ELISA.