ZHOU Xue-Ping, QIAN Xiu-Gong, LIU Yong, XUE Chao-Yang and LI De-Bao. Isolation and characterization of tomato mosaic virus from tomato[J]. Virologica Sinica, 1996, 11(3).
Citation: ZHOU Xue-Ping, QIAN Xiu-Gong, LIU Yong, XUE Chao-Yang, LI De-Bao. Isolation and characterization of tomato mosaic virus from tomato .VIROLOGICA SINICA, 1996, 11(3) : 268.

侵染番茄的番茄花叶病毒的研究

  • 从种传番茄苗中获得一病毒分离物To-Sl,人工摩擦接种7科24种植物,To~Sl能侵染4科15种植物,在番茄上产生花叶,在白肋烟上为局部枯斑。To-Sl的钝化温度为85~90℃,稀释限点为10 ̄(-6)~10 ̄(-7).体外保毒期在一个月以上。病毒粒体杆状,长度主要分布于281~300nm之间,平均长度288nm。病毒衣壳蛋白亚基只有一条多肽链,分子量为21kDa。dsRNA分析测得其基因组长度为6.4kb。琼胎糖双扩散和胶内交叉吸附试验证明,To-Sl与TMV有血清关系,但有一定的差异,病毒粒体电泳分析也表明To-Sl与TMV粒体有差异。在交叉保护试验中,TMV和To-Sl之间均无保护作用。根据以上试验结果To-Sl被鉴定为番茄花叶病毒。这是我国首次系统报道番茄上番茄花叶病毒的侵染。

Isolation and characterization of tomato mosaic virus from tomato

  • virus isolate(To-Sl)from tomato was collected in Ha11gzhou,Zhejiang Province.To-Slcould infect 24 plant species of three families.To-Sl produced mosaic symptoms on tomato,andlocal leision on N.tabocum White Burley. Its thern1al inactivation point was 85~90℃,dilutionend point was 10 ̄(-6)~10 ̄(-7).The virus particles were rod-shaped with the average length of288nm.The molecular weight cf To-Sl coat protein estimated was 2 1 000 Da.dsRNA analysisshowed that To-Sl corLqisted of one single st rand RNA with the length of 6.4 kb.In double-diffusion tests,spur forn1ation was observed between To-Sl and TMV,and the resuIts of in-tragel cross-absorption tests agreed with the double-diffusion tests.In gel electrophoresis,thepatterns of To-Sl virions were different from TMV.To-Sl shared one bands with TMV,butTMV had another band with slow migration.Cross protect ion tests showed To-Sl had no prrvtection eHect to TMV,and vice versa.On the basis of these characteristics theTo-Sl was identi-fied as tomato mosaic virus(ToMV).

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    Isolation and characterization of tomato mosaic virus from tomato

    • 1. Zhejiang Agricultral University

    Abstract: virus isolate(To-Sl)from tomato was collected in Ha11gzhou,Zhejiang Province.To-Slcould infect 24 plant species of three families.To-Sl produced mosaic symptoms on tomato,andlocal leision on N.tabocum White Burley. Its thern1al inactivation point was 85~90℃,dilutionend point was 10 ̄(-6)~10 ̄(-7).The virus particles were rod-shaped with the average length of288nm.The molecular weight cf To-Sl coat protein estimated was 2 1 000 Da.dsRNA analysisshowed that To-Sl corLqisted of one single st rand RNA with the length of 6.4 kb.In double-diffusion tests,spur forn1ation was observed between To-Sl and TMV,and the resuIts of in-tragel cross-absorption tests agreed with the double-diffusion tests.In gel electrophoresis,thepatterns of To-Sl virions were different from TMV.To-Sl shared one bands with TMV,butTMV had another band with slow migration.Cross protect ion tests showed To-Sl had no prrvtection eHect to TMV,and vice versa.On the basis of these characteristics theTo-Sl was identi-fied as tomato mosaic virus(ToMV).

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