为了探讨从核苷酸水平耐汉坦病毒进行分型，设计两对型特异性引物，采用反转录和聚合酶链式反应（ＲＴ－ＰＣＲ），对亚太地区１８株汉坦病毒进行了扩增鉴定，并对其中７株汉坦病毒的ＰＣＲ产物进行了测序分析。ＰＣＲ的分型结果表明，Ⅰ型引物只能扩增血清Ⅰ型病毒的ｃＤＮＡ；Ⅱ型引物也只能扩增血清Ⅱ型病毒，其间无交叉反应。采用巢式ＰＣＲ和限制性内切酶验证了ＰＣＲ产物的特异性。序列分析结果表明，Ｒ３６Ｍ片段Ｇ１区的核苷酸序列与血清Ⅰ型病毒代表株７６－１１８的同源性为７８．４％，而与血清Ⅱ型病毒Ｒ２２的同源性为６８．１％；Ｒ３６与汉坦病毒序列同源性的成对比较结果也表明，Ｒ３６与血清Ⅰ型病毒的同源性均高于血清Ⅱ型病毒；Ｌｅａｋｅｙ虽然能被Ⅱ型引物扩增，但其序列与血清Ⅱ型病毒Ｒ２２的同源性仅为４４．９％，故不属于血清Ⅱ型病毒。上述研究结果表明，反转录聚合酶链反应能对多数汉坦病毒准确分型，但最终结果尚有赖于序列分析。

The present study reports the use of reverse transcriptase polymerase chain reaction(RT-PCR)for typing 18 independently isolated Hantaviruses from Asia-Pacific area by 2 sets of type-specific primers and the sequence analysis.The results of PCR showed that Hantaan specificprimers could amplify all 10 isolates in the Hantaan serotype,while Seoul specific primers werereactive to all 7 isolates in Seoul serotype and Leakey.No cross-reaction was obserred in the ex-periment.The PCR products were analysed by nested PCR and restriction endonuclease digestionfor further confirmation。The sequence analysis of PCR products revealed that the sequence simi-larities of R36 between 76-118 and R22 were 78.4% and 68.1%,respectively. In addition,apairwise comparison of sequence homology revealed that R36 shares a higher degree of sequencehomology with isolates in Hantaan serotype than those in Seoul serotype.Lekey could be ampli-fied by Seoul specific primers