Isolation and Identification of an Avian Reovirus Variant Strain

DIAO Zhen-Fen; XU Wei-Yan; DU Nian-Xin; XUE Heng-Beng

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August 1996

DIAO Zhen-Fen, XU Wei-Yan, DU Nian-Xin and XUE Heng-Beng. Isolation and Identification of an Avian Reovirus Variant Strain[J]. Virologica Sinica, 1996, 11(4).

Citation: DIAO Zhen-Fen, XU Wei-Yan, DU Nian-Xin, XUE Heng-Beng. Isolation and Identification of an Avian Reovirus Variant Strain .VIROLOGICA SINICA, 1996, 11(4) : 352.

禽呼肠孤病毒变异株的分离鉴定

1.
南京农业大学动物医学院郑州牧业工程高等专科学校基础部

摘要

从病鸡肝分离到一株病毒，经电镜检查、理化特性分析、核酸电泳和中和试验等证明它为禽呼肠孤病毒（ＡＲＶ）。该病毒只在鸡胚肝细胞（ＣＥＬｉ）上产生细胞病变（ＣＰＥ），在鸡胚成纤维细胞（ＣＥＦ）和Ｖ＿（ｅｒｏ）细胞上不增殖，它对热无敏感，Ｍｇ￣（２＋）不能增强其对热的稳定性，其基因组中的４个小节段的迁移率与ＡＲＶＦＤＯ和Ｓ＿（１１３３）株明显不同，表明它是不同于ＦＤＯ和Ｓ＿（１１３３）的ＡＲＶ变异株。
- 禽呼肠孤病毒 ARV变异株分离鉴定

Isolation and Identification of an Avian Reovirus Variant Strain

1.
College of Animal Medica1．Nanjing Agriculture University，Nanjing 210095

Abstract

virus strain was isolated from the liver of a sick chicken and identified to be an avian re-ovirus(RV)by observation of electron-microscopy,analysis of physical-chemieal characteris-tics,SDS-PAGE of nucleic acid and neutralization test.The virus strain produced evident cyto-pathic effect(CPE)only on chicken embryo liver cell(CELi)but not on CEF and Vero cell,andwas inactive at 50℃ for 1 hour,even in Mg￣(2+)solution.Additionally,the migration rate of foursmall segments of it's genome was slightly different from ARV FDO and S_(1133) strain.The experi-ment suggested that the virus is a variant strain of ARV.
- Avian reovirus Variant strain Isolation Identification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Identification of an Avian Reovirus Variant Strain

1. College of Animal Medica1．Nanjing Agriculture University，Nanjing 210095

Abstract: virus strain was isolated from the liver of a sick chicken and identified to be an avian re-ovirus(RV)by observation of electron-microscopy,analysis of physical-chemieal characteris-tics,SDS-PAGE of nucleic acid and neutralization test.The virus strain produced evident cyto-pathic effect(CPE)only on chicken embryo liver cell(CELi)but not on CEF and Vero cell,andwas inactive at 50℃ for 1 hour,even in Mg￣(2+)solution.Additionally,the migration rate of foursmall segments of it's genome was slightly different from ARV FDO and S_(1133) strain.The experi-ment suggested that the virus is a variant strain of ARV.