TIEN Po. Sequence Homology and Base Pairing between Cucumber M osaic VirusSatellite RNA an d Potato Spindle Tube r Viroid[J]. Virologica Sinica, 1996, 11(4).
Citation:
TIEN Po.
Sequence Homology and Base Pairing between Cucumber M osaic Virus
Satellite RNA an d Potato Spindle Tube r Viroid .VIROLOGICA SINICA, 1996, 11(4)
: 378.
黄瓜花叶病毒卫星RNA与马铃薯纺锤形块茎类病毒间序列同源性与碱基配对
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摘要
1983年田波等首次报道了应用病毒卫星RNA防治黄瓜花叶病毒(CMV)获得成功。将致弱病毒的卫星RNA加入协助病毒基因组中柯建一种新的致弱病毒。称谓卫星RNA生防制剂(BCA) 用BCA接种青椒、番茄等的幼苗，可保护其植株免受田间野生病毒的侵染[2-3]。最近我们的实验证明，BCA预先接种的和表达卫星RNA的转基因番茄植株接种马铃薯纺锤形块茎类病毒(PsTvd)时。显著降低了PSTVd的症状和组织内PSTVd的积累“]。为了探索有病毒卫星RNA的植物抵抗PSTVd的机理，我们比较了CMV Sat—RNA和PSTVd核背酸序列相关性。发现其问有47．8-66．7％同荔f性。井能形成长度为1O～12碱基的6个碱基配对区。为其抗病机理提供了一种可能的解释。
Sequence Homology and Base Pairing between Cucumber M osaic Virus
Satellite RNA an d Potato Spindle Tube r Viroid
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Abstract
The results of computer analysis showed that，(1)47．8～66．7％ seq homolo~ between CARNA5D (t-)RNA and PS『rVld(+)RNA；(2)48．1～63．4％ sequeEtce homology be．tween CARNA5D(4-)RNA and PSTVd (一)RNA；(3)six dotplot between CARN^5D(+)RNA and PSTV_d(+)RNA；and(4)six base pairing regions between CARNASD(+)RNA and P明、Vd(4-)RNA containing high G ：C ratio and high△ G values：KJ／tool= 一872．4～一975．9．
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References
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Proportional views
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