Study on the gene of poliovirus type I isolated from environmental
water by PCR

DIAO Wen-Ban; TUN Tao-Lin; LI Jia-Fu; FANG Zhao-Yin; WANG Qiu-Gong; ZHANG Jing

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February 1997

DIAO Wen-Ban, TUN Tao-Lin, LI Jia-Fu, FANG Zhao-Yin, WANG Qiu-Gong and ZHANG Jing. Study on the gene of poliovirus type I isolated from environmentalwater by PCR[J]. Virologica Sinica, 1997, 12(1).

Citation: DIAO Wen-Ban, TUN Tao-Lin, LI Jia-Fu, FANG Zhao-Yin, WANG Qiu-Gong, ZHANG Jing. Study on the gene of poliovirus type I isolated from environmental water by PCR .VIROLOGICA SINICA, 1997, 12(1) : 26.

PCR法检测外环境水中脊髓灰质炎病毒Ⅰ型基因的研究

1.
连云港市卫生防疫站中国预防医学科学院病毒学研究所

摘要

应用聚合酶链反应（PCR）技术检测外环境水中分离的脊髓灰质炎病毒Ⅰ型（PVⅠ）毒株基因型，发现所分离的22株病毒．均为疫苗SabinⅠ相关株．脊髓灰质炎病毒的温度敏感（T特征）试验亦提示为弱毒株，与PCR试验相吻合。表明在实施强化免疫和常规免疫后，外环境中的脊髓灰质炎病毒已被疫苗相关株循环所取代。同时证实用PCR作为环境中PVⅠ病毒株的基因型分析的检测方法是特异和敏感的。
- 脊髓灰质炎病毒 SabinⅠ疫苗相关株聚合酶链反应温度敏感试验

Study on the gene of poliovirus type I isolated from environmental water by PCR

1.
Lianyungang City Sanitary and Anti—epidemic station，Lianyungang 222003

Abstract

The genes of poliovirus type I isolated from environmental water were studied by PCR (polymerase chain reaction). It was found that 22 strains of the virus were all Sabin-related. The result showed that the wild polioviruses in the environmental water were substituted by the sabinstrain completely after carrying on the conduct of mass immunization campaignS and EPI in thisarea. The method was suitable for the gene analysis of poliovirus type I.
- Poliovirus Sabin I-related isolation Polymerase chain reaction Sensitivity test oftemperature

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the gene of poliovirus type I isolated from environmental water by PCR

1. Lianyungang City Sanitary and Anti—epidemic station，Lianyungang 222003

Abstract: The genes of poliovirus type I isolated from environmental water were studied by PCR (polymerase chain reaction). It was found that 22 strains of the virus were all Sabin-related. The result showed that the wild polioviruses in the environmental water were substituted by the sabinstrain completely after carrying on the conduct of mass immunization campaignS and EPI in thisarea. The method was suitable for the gene analysis of poliovirus type I.