JIANG Li-Fang, JIANG Zhen-You and GUO Hui-Yu. Study on dengue virus infection of human endothelial cells[J]. Virologica Sinica, 1997, 12(1).
Citation: JIANG Li-Fang, JIANG Zhen-You, GUO Hui-Yu. Study on dengue virus infection of human endothelial cells .VIROLOGICA SINICA, 1997, 12(1) : 32.

登革病毒对人血管内皮细胞感染性的研究

  • 用登革病毒Ⅱ型(DV2)感染体外培养和传代的人脐静脉内皮细胞(HUVEC),研究发现,HUVEC是登革病毒的允许性细胞。病毒感染后12h即可在培养上清中用微量蚀斑法测出病毒,病毒滴度48h达高峰,以后迅速下降。并发现在一定范围内病毒产量随病毒感染复数(MOI)的增加而增高。间接免疫荧光法证明感染的HUVEC胞浆及胞膜上携带DV2抗原。电镜和光镜下,感染细胞未见明显的形态和结构改变。

Study on dengue virus infection of human endothelial cells

  • The replication of dengue-2 virus in cultured human endothelial cells derived from humanumbilical veins was demonstrated. The viruses were detected in the supernatant as early as 12 h after infection by microplaque forming assay on C6/36 monolayer cells. The highest viral titers were obtained at 48h, then rapidly declined to very low titers at 96h. Dengue-2 virus antigenpositive human endothelial cell were demonstrated by an indirect immunofluorescent assay. No obvious differences in morphology and structure were observed between the virus infected cells and uninfected cells under phase-contrast microscope and transmission electron microscope. Viral production of infeted cells was depended on the multiplicity of infection (MOI). As the viral dose was increased from MOI of 0.1 PFU/cell to 100 PFU/cell, significant increase of viral production was observed.

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    Study on dengue virus infection of human endothelial cells

    • 1. 登革病毒Ⅱ型DV2 人脐静脉内皮细胞HUVEC 感染

    Abstract: The replication of dengue-2 virus in cultured human endothelial cells derived from humanumbilical veins was demonstrated. The viruses were detected in the supernatant as early as 12 h after infection by microplaque forming assay on C6/36 monolayer cells. The highest viral titers were obtained at 48h, then rapidly declined to very low titers at 96h. Dengue-2 virus antigenpositive human endothelial cell were demonstrated by an indirect immunofluorescent assay. No obvious differences in morphology and structure were observed between the virus infected cells and uninfected cells under phase-contrast microscope and transmission electron microscope. Viral production of infeted cells was depended on the multiplicity of infection (MOI). As the viral dose was increased from MOI of 0.1 PFU/cell to 100 PFU/cell, significant increase of viral production was observed.

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