PAN Wei, FANG Fang, CUI Xiao-Hong, SONG Yan-Bin and CU Zhong-Tian. Detection and sequencing of hepatitis C virus RNA in blood donors without anti - HCV igG anti- bodies[J]. Virologica Sinica, 1997, 12(1).
Citation: PAN Wei, FANG Fang, CUI Xiao-Hong, SONG Yan-Bin, CU Zhong-Tian. Detection and sequencing of hepatitis C virus RNA in blood donors without anti - HCV igG anti- bodies .VIROLOGICA SINICA, 1997, 12(1) : 38.

抗-HCV阴性献血员中丙型肝炎病毒RNA检测及序列分析

  • 对95份抗—HCVIgG阴性献血员采用逆转录聚合酶链反应法(PCR)检测丙型肝炎病毒RNA,结果8次中有6次其检测出17份阳性标本(17/95,17.9%),复查抗—HCVIgG仍为阴性。对其中8份阳性产物中高变区1的序列分析结果表明均为不同株HCV序列.排除了PCR污染的可能性。对其中2份阳性产物测定了全序列并与HCV各基因型代表株的相应序列比较,与HCVⅡ型相应序列的核苷酸同源性为77%~79%。而与HCVⅠ、Ⅲ、Ⅳ相应序列间的同源性为62%~69%,表明为HCVⅡ型序列。结果提示献血员抗—HCVIgG筛选不能完全排除HCV感染者,漏检不是由于HCV基因序列变异.而是检测方法本身缺陷所致。

Detection and sequencing of hepatitis C virus RNA in blood donors without anti - HCV igG anti- bodies

  • Reverse transcription polymerase chain reaction was used to detect hepatitis C virus (HCV) RNA in 95 serum specimens of blood donors with anti-HCV IgG negative. Six out of 8 detections, HCV RNA was detected in 17 of 95 specimens (17. 9% ). These 17 HCV RNA positive specimens were detected once more for anti HCV IgG to confirm their negative detection. Sequencing data of hypervariable region from 8 of 17 positive PCR products showed that the amplificated sequences came from different HCV strains, and were not the false amplification because of PCR contamination. In these 8 PCR products, two nucleotide sequences were determined at fulllength, and showed higher homology of 77%~79% with the corresponding sequences of representative strain of HCV genotype Ⅱ than that of 62%~69% with the sequences of HCV genotypes Ⅰ, Ⅲ, Ⅳ, and were verified as HCV genotype Ⅱ sequences which are most popular among Chinese HCV infected cases. Our data demonstrated that anti-HCV IgG detection is not sensitive enough for HCV scre

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    Detection and sequencing of hepatitis C virus RNA in blood donors without anti - HCV igG anti- bodies

    • 1. Dep. of Microbiology,Second Medical University

    Abstract: Reverse transcription polymerase chain reaction was used to detect hepatitis C virus (HCV) RNA in 95 serum specimens of blood donors with anti-HCV IgG negative. Six out of 8 detections, HCV RNA was detected in 17 of 95 specimens (17. 9% ). These 17 HCV RNA positive specimens were detected once more for anti HCV IgG to confirm their negative detection. Sequencing data of hypervariable region from 8 of 17 positive PCR products showed that the amplificated sequences came from different HCV strains, and were not the false amplification because of PCR contamination. In these 8 PCR products, two nucleotide sequences were determined at fulllength, and showed higher homology of 77%~79% with the corresponding sequences of representative strain of HCV genotype Ⅱ than that of 62%~69% with the sequences of HCV genotypes Ⅰ, Ⅲ, Ⅳ, and were verified as HCV genotype Ⅱ sequences which are most popular among Chinese HCV infected cases. Our data demonstrated that anti-HCV IgG detection is not sensitive enough for HCV scre

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