Cloning and sequencing of NS5 partial gene from HGV China isolate

ZHOU Yo-Sen; CHEN Wei; WANG Hai-Tao; ZHAO Qiu-Min; XU Jing; WANG Jing

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February 1997

ZHOU Yo-Sen, CHEN Wei, WANG Hai-Tao, ZHAO Qiu-Min, XU Jing and WANG Jing. Cloning and sequencing of NS5 partial gene from HGV China isolate[J]. Virologica Sinica, 1997, 12(1).

Citation: ZHOU Yo-Sen, CHEN Wei, WANG Hai-Tao, ZHAO Qiu-Min, XU Jing, WANG Jing. Cloning and sequencing of NS5 partial gene from HGV China isolate .VIROLOGICA SINICA, 1997, 12(1) : 50.

庚型肝炎病毒(HGV)NS5区部分基因的克隆和cDNA序列测定

1.
军事医学科学院微生物流行病研究所军事医学科学院微生物流行病研究

摘要

用RT－PCR技术从一例献血员血清标本中扩增出HGV非结构区的部分基因片段，克隆后测定。cDNA序列。该序列与国外三株HGV的核苷酸同源性在90％以上；与GBVA和GBVB的同源性分别为44．7％和33．17％；与已发表的23个HCV全序列的相应序列比较，同源性均小于40％。结果表明，克隆的病毒株为HGV中国株。同时，用RTPCR检测了河北固安和南京地区的115份HCV感染者标本．HGVRNA阳性率为3．47％。表明我国某些特殊人群中HGV感染率较高，是一个不容忽视的问题。
- 庚型肝炎病毒 cDNA序列反转录聚合酶链反应

Cloning and sequencing of NS5 partial gene from HGV China isolate

1.
1nstitude of Microbiology and Epidemiology．Beijing 100071

Abstract

A partial gene of HGV was cloned and sequenced from the serum of a blood donor in Hebei Province of China. The partial sequence from the nostructural region gene showed 90. 5% nucleotide identity and 95 % amino acid identity over the corresponding region of isolates in America. One hundred and fifteen samples collected in Jangshu and Hebei Provinces were detected for HGV RNA by RT-PCR. The HGV RNA positive rate is 3.47%.
- Hepatitis G virus PCR cDNA sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and sequencing of NS5 partial gene from HGV China isolate

1. 1nstitude of Microbiology and Epidemiology．Beijing 100071

Abstract: A partial gene of HGV was cloned and sequenced from the serum of a blood donor in Hebei Province of China. The partial sequence from the nostructural region gene showed 90. 5% nucleotide identity and 95 % amino acid identity over the corresponding region of isolates in America. One hundred and fifteen samples collected in Jangshu and Hebei Provinces were detected for HGV RNA by RT-PCR. The HGV RNA positive rate is 3.47%.