自1979年Provost体外培养甲型肝炎病毒（HAV）首获成功以来［1］，有关HAV对多种理化因子抗力的研究不少【1，但对其具体作用机制，迄今尚未见诸报道。为此，本研究通过观察UV照射对HAVRNA链与衣壳蛋白完整性等的影响，探讨UV对HAV的灭活机理，为灭活HAV的实际应用提供?..

This study showed that 300 W/cm2 UV irradiation for 5 min could inactivate 94.63% ofHAV. No obvious changes were found experimentally under this irradiation dose, other than the decrease of RNA transfection ability. After 20 min UV irradiation, all of HAV were inactivated. For the RNA sedimentation, the original single UV absorption peak (32.9S) became two (11 S and 15.2S) after the irradiation. The fragment of 5'-untranslated region (HAV RNA 222~ 819) could not be detected with RT-PCR. The RNA transfection became negative. But the fragment of P1 region (HAV RNA 2823~3049) could still be detected with RT-PCR, the hybridhation shadow of 3'-terminal poly(A) tail with oligo(dT) probe labelled with -(32)P also became slightly lighter, and no obvious changes were found in the antigenicity of HAV detectedwith ELISA and in 4 capsid polypeptides which was separated by 15% SDS-polyacrylamide gel containing 4 mol/L urea.