Dynamic observation on proliferation of HFRSV in chigger mite

ZHANG Yun; LI Xian-Fu; WU Guang-Hua; ZHANG Jia-Ju; JIANG Ke-Jian

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April 1997

ZHANG Yun, LI Xian-Fu, WU Guang-Hua, ZHANG Jia-Ju and JIANG Ke-Jian. Dynamic observation on proliferation of HFRSV in chigger mite[J]. Virologica Sinica, 1997, 12(2).

Citation: ZHANG Yun, LI Xian-Fu, WU Guang-Hua, ZHANG Jia-Ju, JIANG Ke-Jian. Dynamic observation on proliferation of HFRSV in chigger mite .VIROLOGICA SINICA, 1997, 12(2) : 132.

肾综合征出血热病毒在恙螨体内增殖的动态观察

1.
南京军区军事医学研究所陕西省卫生防疫站

摘要

取饲养的小盾纤恙螨幼虫和若虫．每20d为一批制成无菌滤液接种Vero-E6细胞测定HFRSV滴度，动态观察HFRSV在螨体内的增殖情况，并用PCR技术检测恙螨幼虫和若虫体内HFRSV－RNA。结果如下：小盾纤恙螨幼虫期除60d一批外，其余各批均在不同时间内测出HFRSV滴度，3批若虫中亦有2批测出HFRSV滴度并检测到HFRSV－RNA。结果为小盾纤恙螨作为HFRS的传播媒介提供了进一步的证据。
- 小盾纤恙螨肾综合征出血热病毒增殖

Dynamic observation on proliferation of HFRSV in chigger mite

1.
Nanjing Institute of Military Medecine．Nanjing 210002

Abstract

In order to observe further dynamic changes on proliferation of HFRSV in Leptotrombidium Scutellare, the raised Leptotrombidium scutellare were ground and sterilized with an interval of 20-40 days, the filterate of each batch was inoculated separately into Vero - E6 cells, titres of HFRSV and HFRSV-RNA were detected. The results confirmed that HFRSV were positive in most batches of larva. The above results indicated that the Leptotrombidlum sutellare is a transmitting vector of HFRSV.
- Leptotrombidium Sutellare Hemorrhagic Fever with Renal Syndrome Virus Pro-liferation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Dynamic observation on proliferation of HFRSV in chigger mite

1. Nanjing Institute of Military Medecine．Nanjing 210002

Abstract: In order to observe further dynamic changes on proliferation of HFRSV in Leptotrombidium Scutellare, the raised Leptotrombidium scutellare were ground and sterilized with an interval of 20-40 days, the filterate of each batch was inoculated separately into Vero - E6 cells, titres of HFRSV and HFRSV-RNA were detected. The results confirmed that HFRSV were positive in most batches of larva. The above results indicated that the Leptotrombidlum sutellare is a transmitting vector of HFRSV.