为了解国CBV-C/HCV株的基因序列与国外分离株的同源性状况，对我国GBV-C/HCV的5’端非编码区（5’NCR）。cDNA进行了序列测定。参考国外发表的序列资料，在相对保守的5’NCR设计两对HGV特异性引物。采用热变性法提取云南省一个静脉吸毒者血浆中的GBV－C／HGVRNA逆转录为cDNA后进行巢式聚合酶链反应（PCR）扩增，获得238bp的片段。PCR产物纯化后直接经双脱氧链末端终止法测定核苷酸序列。与国外分离株比较，同源性为86.36％～90.9t％，微分区域变异较大。结果表明，所扩增片段属于GBV－C／HGV基因，所测序列可为引物设计据供依据。对核酸变异性分析有一定意义。

The cDNA sequence of 5' non - coding region (5'NCR) of GBV - C/HGV strain from China was determined. According to the previously reported sequences of HGV, two pairs of primers specific for HGV from relatively conserved regha in the 5' NCR was designed- GBV - C/HGV RNA derived from the plasma of a intravenous drug user in Kunming district (Yunnan Province,China) was extracted using the heat denaturization method and was subsequently converted to cD-NA by reverse transcription before nested RCR. The amplified product with 238 bp was purified and then directly sequenced by dideoxy nucleotide chain termination method. A Comarison of the nucleotlde sequence of GBV - C/HGV from China with that of several previously reported isolates from abroad showed the homology to be 86. 36 % ~ 90. 91 % and a divergent region. The results demonstrated that the amplified fragment was derived from the genome of GBV - C/HGV. The sequence of the GBV - C/HGV 5' NCR determined here was useful for the selection of primers and for the