Specific binding of hepatitis C virus to the Fc fragment of IgG molecules

HAN Jian-Qiu; WU Ping; WarrenN．Schmidt; Pao-minLoh; GaryNeil; DouglasR．LaBrecque; JackT．Stapleton

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June 1997

HAN Jian-Qiu, WU Ping, WarrenN．Schmidt, Pao-minLoh, GaryNeil, DouglasR．LaBrecque and JackT．Stapleton. Specific binding of hepatitis C virus to the Fc fragment of IgG molecules[J]. Virologica Sinica, 1997, 12(3).

Citation: HAN Jian-Qiu, WU Ping, WarrenN．Schmidt, Pao-minLoh, GaryNeil, DouglasR．LaBrecque, JackT．Stapleton. Specific binding of hepatitis C virus to the Fc fragment of IgG molecules .VIROLOGICA SINICA, 1997, 12(3) : 244.

丙型肝炎病毒与人IgG Fc片段的特异性结合

1.
卫生部上海生物制品研究所 DepartmentofInternalMedicine TheUniversityoflowaCollegeofMedicine lowaCity52246 U．S．A

摘要

使用亲和捕获PCR法研究丙型肝炎病毒（HCV）和人IgG的相互作用，发现HCV能与IgG及完整的IgGFc片段结合，但不与Fab片段结合。这种结合可以被Fc片段所竞争抑制，而不能被Fab片段所抑制。HCVRNA阴性血浆不能阻断Fc片段与HCV的结合，推测这是一种HCV颗粒与IgGFc片段之间的特异性结合。本研究揭示了HCV与IgG之间的相互作用，探讨了HCV引起免疫复合物疾病的机理及丙型肝炎的慢性化倾向。
- 丙型肝炎病毒 PCR IgG 免疫复合物

Specific binding of hepatitis C virus to the Fc fragment of IgG molecules

1.
卫生部上海生物制品研究所 DepartmentofInternalMedicine TheUniversityoflowaCollegeofMedicine lowaCity52246 U．S．A

Abstract

Affinity- capture PCR method was adapted to study the interaction between hepatitis C virus (HCV) and human IgG, and demonstrated that HCV bound IgG and Fc fragment but not Fab fragment, The binding could be inhibited cometitively by Fc fragment but not Fab fragment. Meanwhile, pre -mccubation with HCV - RNA negative plapa did not inhibit subsequent binding of HCV to Fc fragment, further confirmed that this binding is just between HCV particles and Fc fragment, The results demonstrated an interaction betwee HCV and IgG, and gave a new explanaion by which HCV may trigger immune complex disease and the chronic trend of hepatitis C.
- Hepatitis C virus PCR IgG Immunecomlex

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Specific binding of hepatitis C virus to the Fc fragment of IgG molecules

1. 卫生部上海生物制品研究所 DepartmentofInternalMedicine TheUniversityoflowaCollegeofMedicine lowaCity52246 U．S．A

Abstract: Affinity- capture PCR method was adapted to study the interaction between hepatitis C virus (HCV) and human IgG, and demonstrated that HCV bound IgG and Fc fragment but not Fab fragment, The binding could be inhibited cometitively by Fc fragment but not Fab fragment. Meanwhile, pre -mccubation with HCV - RNA negative plapa did not inhibit subsequent binding of HCV to Fc fragment, further confirmed that this binding is just between HCV particles and Fc fragment, The results demonstrated an interaction betwee HCV and IgG, and gave a new explanaion by which HCV may trigger immune complex disease and the chronic trend of hepatitis C.