Expression of Hantaan Virus Envelope Glyeoproteins Gl and G2 in E .coli

HUANG Chang-Xing; YANG Wei-Song; HANG Chang-Shou; BAI Xue-Fan; LI Guang-Yu

PDF
Cite
Share

facebook

twitter

google

linkedin

August 1997

HUANG Chang-Xing, YANG Wei-Song, HANG Chang-Shou, BAI Xue-Fan and LI Guang-Yu. Expression of Hantaan Virus Envelope Glyeoproteins Gl and G2 in E .coli[J]. Virologica Sinica, 1997, 12(4): 313-321.

Citation: HUANG Chang-Xing, YANG Wei-Song, HANG Chang-Shou, BAI Xue-Fan, LI Guang-Yu. Expression of Hantaan Virus Envelope Glyeoproteins Gl and G2 in E .coli .VIROLOGICA SINICA, 1997, 12(4) : 313-321.

在大肠杆菌中分别表达汉滩病毒囊膜糖蛋白G1和G2

1.
第四军医大学唐都医院传染科，西安710038

摘要

要利用PCR方法扩增了汉濉病毒76118株囊膜糖蛋白G1和G2的编码区基因，并将 PCR产物克隆到T一载体中，用限制性内切酶将G1和G2的编码区基因切下．并克隆到表达载体 pBV220中掏建G1和G2的表达质粒。诱导表达后在SDSPAGE凝胶中未见表达产物带，表达的G1和G2能与部分抗Gl和G2的单克隆抗体发生反应．但用Westexn～blot方法不能检测到表达产物。用表达的G1和G2免疫小白鼠能刺激小白鼠产生特异性抗汉滩病毒的抗体，同接免疫荧光抗体的滴度可分别达到1：160和1：320。
- 汉坦病毒
- , 囊膜糖蛋白G1
- , 囊膜糖蛋白G2
- , 表达

Expression of Hantaan Virus Envelope Glyeoproteins Gl and G2 in E .coli

1.
Tangdu Hospital ，The Fouth Mititary Medical

Abstract

Tlle expression vector plasmid pBV220 was used tO expre~ ,the envelope曹lycuproteins，G1 andG2 ofFtantsall~vil-uJ5inE．co／i．First，the codingframegenesofG1 andG2werem plifiedby PCR，and doned into the PCR product cloning Tvector，then they；were cut f u蜕|唱抟s韵曲蛹n endonudeases from the T～vector cloning plasmids．Last．the expression pl of G1^ G2 were constixtcted llgatingthe oondingframesofG1 andG2with眈曲iDn豫呲蛐．The expressed proteins could not be seen on SDSPAGE ge1．Tbey coutd he reacted with part of mono clonal antibodies against Hantaan virus G1 and G2．but not he t墨ted in We|tem ，bI卧．Mice were immunized with expressed Gl and G2．an tibody ag ainst Han ~ n V~t／S。0uld he ~sted in the sera of the immunized mice．the IFA(Indirect 1：160 and l：320，respectively． Assay)antibody titers were l
- Hantaan virus
- , Envelope glycoprotein G1
- , Envelope glycoprotein G2
- , Expression

References
Proportional views

PDF

Article Metrics

Article views(3928) PDF downloads(795) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of Hantaan Virus Envelope Glyeoproteins Gl and G2 in E .coli

1. Tangdu Hospital ，The Fouth Mititary Medical

Abstract: Tlle expression vector plasmid pBV220 was used tO expre~ ,the envelope曹lycuproteins，G1 andG2 ofFtantsall~vil-uJ5inE．co／i．First，the codingframegenesofG1 andG2werem plifiedby PCR，and doned into the PCR product cloning Tvector，then they；were cut f u蜕|唱抟s韵曲蛹n endonudeases from the T～vector cloning plasmids．Last．the expression pl of G1^ G2 were constixtcted llgatingthe oondingframesofG1 andG2with眈曲iDn豫呲蛐．The expressed proteins could not be seen on SDSPAGE ge1．Tbey coutd he reacted with part of mono clonal antibodies against Hantaan virus G1 and G2．but not he t墨ted in We|tem ，bI卧．Mice were immunized with expressed Gl and G2．an tibody ag ainst Han ~ n V~t／S。0uld he ~sted in the sera of the immunized mice．the IFA(Indirect 1：160 and l：320，respectively． Assay)antibody titers were l