Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E ．coli with Differential Expression Vector

HUANG Chang-Xing; YANG Wei-Song; HANG Chang-Shou; BAI Xue-Fan; LI Guang-Yu

PDF
Cite
Share

facebook

twitter

google

linkedin

August 1997

HUANG Chang-Xing, YANG Wei-Song, HANG Chang-Shou, BAI Xue-Fan and LI Guang-Yu. Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E ．coli with Differential Expression Vector[J]. Virologica Sinica, 1997, 12(4): 322-328.

Citation: HUANG Chang-Xing, YANG Wei-Song, HANG Chang-Shou, BAI Xue-Fan, LI Guang-Yu. Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E ．coli with Differential Expression Vector .VIROLOGICA SINICA, 1997, 12(4) : 322-328.

用不同载体在大肠杆菌中表达汉滩病毒囊膜糖蛋白G1和G2

1.
第四军医大学唐都医院传染科，西安710038

摘要

为提高汉滩病毒囊膜糖蛋白G1和G2的表达水平，利用两种不同的表达载体pKK223一 3和pGEX一4T 1构建G1和G2的表达质粒．其中pGEX一4T一1为融合蛋白表达载体。诱导所构建的G1和G2表遗质粒表达G1和G2．用表达的G1和G2免疫小白鼠诱导产生的抗汉滩病毒特异性的间接免疫荧光抗体摘度无明显差别，从而说明表达载体对汉滩病毒G1和G2在大肠杆菌中的表达水平影响不太．表达水平都较低。同时，利用pGEX一4T一1构建G1和G2的部分多肽的表达质粒，但表达水平较完整的G1和( 2无明显提高。
- 汉坦病毒
- , 囊膜糖蛋白G1
- , 囊膜糖蛋白G2
- , 表达

Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E ．coli with Differential Expression Vector

1.
Tangdu Haspital ，The Fourth Military Medical University ,Xi'an 710038,China

Abstract

For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E．coli, the expression vector pKK223-3 and pGEX-4T -1 were used for constructingGl and G1 expression plasmids， pGEX-4T-l was a fusion-protein expression vector phsmid．The results showed that the expression levels of hantaan virus envelope glycoproteins G1 a nd G2 w ith differential expression vector plasmids were riot ohvions different．The exprssion levels of the portion of GI andG2 proteinswere about the same with the complete G1 and G2．
- Hantaan virus
- , Envelope glycopmtein G1
- , Envelope glycoproteln G2
- , Expression

References
Proportional views

PDF

Article Metrics

Article views(4118) PDF downloads(993) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E ．coli with Differential Expression Vector

1. Tangdu Haspital ，The Fourth Military Medical University ,Xi'an 710038,China

Abstract: For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E．coli, the expression vector pKK223-3 and pGEX-4T -1 were used for constructingGl and G1 expression plasmids， pGEX-4T-l was a fusion-protein expression vector phsmid．The results showed that the expression levels of hantaan virus envelope glycoproteins G1 a nd G2 w ith differential expression vector plasmids were riot ohvions different．The exprssion levels of the portion of GI andG2 proteinswere about the same with the complete G1 and G2．