Establishment of a M armoset Lymphoblastoid Cell Line Releasing Transforming Epstein Barr Virus

SUN Xiao-Mei; LIANG Wu-Sheng; DAI Jie-Jie; GAO Jia-Hong

PDF
Cite
Share

facebook

twitter

google

linkedin

February 1998

SUN Xiao-Mei, LIANG Wu-Sheng, DAI Jie-Jie and GAO Jia-Hong. Establishment of a M armoset Lymphoblastoid Cell Line Releasing Transforming Epstein Barr Virus[J]. Virologica Sinica, 1998, 13(1).

Citation: SUN Xiao-Mei, LIANG Wu-Sheng, DAI Jie-Jie, GAO Jia-Hong. Establishment of a M armoset Lymphoblastoid Cell Line Releasing Transforming Epstein Barr Virus .VIROLOGICA SINICA, 1998, 13(1) : 33.

一株产生EB病毒的狨猴淋巴细胞株的建立

1.
中国协和医科大学医学生物学研究所

摘要

用Ｂ９５８细胞株产生的ＥＢ病毒，转化棉顶狨猴外周血淋巴细胞，获得ＫＭＴ３细胞株。该细胞株能产生高滴度的ＥＢ病毒。含ＥＢ病毒衣壳抗原的自然阳性细胞率为３～５％，激活后达５０～６０％，其培养上清液可直接转化人淋巴细胞得到传代细胞系。ＫＭＴ３细胞染色体数２ｎ＝４６条
- 淋巴细胞 EB病毒转化细胞株棉顶狨猴

Establishment of a M armoset Lymphoblastoid Cell Line Releasing Transforming Epstein Barr Virus

1.
Lymphoblastoid Epstein Barr Virus Transformation Cell line Cotton top tamarin

Abstract

A lymphoblastoid cell line (KMT 3) was established by EBV induced B cell transformation in the cotton top tamarin. The viruses were prepared from the culture fluid of B 95 8 cells by passing through a 0.45μm filter. KMT 3 can produce high titer infections EB viruses. Its VCA positive cell rate was 3～5% in natural and 50～60% after activation. Human B lymphocytes could be immortalized directly from KMT 3 supernatant. The number of chromosomes was 46.
- Lymphoblastoid Epstein Barr Virus Transformation Cell line Cotton top tamarin

References
Proportional views

PDF

Article Metrics

Article views(4118) PDF downloads(844) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Establishment of a M armoset Lymphoblastoid Cell Line Releasing Transforming Epstein Barr Virus

1. Lymphoblastoid Epstein Barr Virus Transformation Cell line Cotton top tamarin

Abstract: A lymphoblastoid cell line (KMT 3) was established by EBV induced B cell transformation in the cotton top tamarin. The viruses were prepared from the culture fluid of B 95 8 cells by passing through a 0.45μm filter. KMT 3 can produce high titer infections EB viruses. Its VCA positive cell rate was 3～5% in natural and 50～60% after activation. Human B lymphocytes could be immortalized directly from KMT 3 supernatant. The number of chromosomes was 46.