ZHANG Yun, ZHU Jin, LI Xian-Fu, TANG Jia-Qi, WU Guang-Hua, ZHANG Jia-Ju and ZHOU Yan-Ping. Detection of hemorrhagic fever with renal syndrome virus RNA in chigger mite by in situ hybridization[J]. Virologica Sinica, 1998, 13(1).
Citation: ZHANG Yun, ZHU Jin, LI Xian-Fu, TANG Jia-Qi, WU Guang-Hua, ZHANG Jia-Ju, ZHOU Yan-Ping. Detection of hemorrhagic fever with renal syndrome virus RNA in chigger mite by in situ hybridization .VIROLOGICA SINICA, 1998, 13(1) : 44.

原位分子杂交法检测恙螨体内肾综合征出血热病毒核酸的研究

  • 为探讨肾综合征出血热病毒(HFRSV)在恙螨体内的分布和定位,采用原位分子杂交技术检测恙螨体内HFRSVRNA。结果发现:原位分子杂交技术的检出阳性率较IFAT高;HFRSV阳性信号颗粒呈弥散分布,多见于恙螨幼虫和若虫的腹部组织细胞内,该部位是恙螨的卵巢细胞、中肠及支囊上皮细胞。前体组织细胞内少见;个别幼虫和若虫的前足和中足细胞内亦可见到HFRSVRNA阳性信号。在原位分子杂交中,若虫组织细胞内的HFRSVRNA阳性信号较幼虫密集而且量多,表明HFRSV在恙螨体内可传递并有增殖现象

Detection of hemorrhagic fever with renal syndrome virus RNA in chigger mite by in situ hybridization

  • In order to study the distribution and position of HFRSV in chigger mite, in situ hybridization was used for detection of hemorrhagic fever with renal syndrome virus (HFRSV) RNA.The results showed that positive rate of HFRSV by in situ hybridization was higher than that by IFAT. Positive signals were observed mostly in belly cells of larva and nymph. The positions were probably ovary cells and midgut. A few positive signals were seen in foreleg and middle leg cells also. The positive signals of HFRSV in nymph were much more than in larva. It demonstrated that HFRSV can be transmitted by transstadial and proliferate in mites.

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    Detection of hemorrhagic fever with renal syndrome virus RNA in chigger mite by in situ hybridization

    • 1. Detection of Hemorrhagic Fever with Renal Syndrome Virus RNAin Chigger Mite by in situ Hybridization

    Abstract: In order to study the distribution and position of HFRSV in chigger mite, in situ hybridization was used for detection of hemorrhagic fever with renal syndrome virus (HFRSV) RNA.The results showed that positive rate of HFRSV by in situ hybridization was higher than that by IFAT. Positive signals were observed mostly in belly cells of larva and nymph. The positions were probably ovary cells and midgut. A few positive signals were seen in foreleg and middle leg cells also. The positive signals of HFRSV in nymph were much more than in larva. It demonstrated that HFRSV can be transmitted by transstadial and proliferate in mites.

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