RONG Guang-Ya, SUN Jie, ZHOU Ji-Wen, LIN Ji-Ming and ZHANG Fang. Preparation and Application of the New Type Hepatitis E Virus Diagnostic Kit[J]. Virologica Sinica, 1998, 13(1).
Citation: RONG Guang-Ya, SUN Jie, ZHOU Ji-Wen, LIN Ji-Ming, ZHANG Fang. Preparation and Application of the New Type Hepatitis E Virus Diagnostic Kit .VIROLOGICA SINICA, 1998, 13(1) : 69.

新型戊型肝炎诊断试剂盒的研制及其应用

  • 用HEVORF3合成肽及ORF2重组抗原研制成新型HEVEIA诊断试剂盒。与GenlabsHEVEIA检测比较,灵敏度和特异性均达100%(60/60)。三批试剂精密性测定均<10%。该试剂盒置4℃8个月或37℃4d保持稳定。检测不同肝炎患者HEV抗体,发现急性非甲非乙非丙肝炎中有63.2%,甲肝有13.4%,乙肝有8.3%,丙肝有6.6%,正常人群为2.9%。所研制的戊型肝炎诊断试剂盒,灵敏度高,特异性强,精密性好,稳定性合格。适用于戊型肝炎诊断及戊肝病毒感染的流行病学调查

Preparation and Application of the New Type Hepatitis E Virus Diagnostic Kit

  • Solid phase enzyme immunoassay (EIA) kit was developed for detecting anti HEV IgG by using synthetic peptide from the open reading frame (ORF)3 and recombinant antigens from ORE2.When the kit was applied to detect HEV antibodies in sera of clinical patients the results were quite consistent with the HEV diagnostic kit from Genlabs.The consistent rate reached 100% (60/60).CV of three lot kit was less than 10%.The kit was stable for 8 months at 4℃or for 4 days at 37℃.The kit was used to detect anti HEV IgG among clinically different patients with acute Non A,Non B,Non C hepatitis,Hepatitis A,Hepatitis B,Hepatitis C and normal human group,the positive rates were 63.2%,13.4%,8.3%,6.6%,2.9%, respectively. The date indicated that the HEV EIA kit is sensitive,specificic and stable,may be used to detect antibody of Hepatitis E Virus among patients and normal human group.

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    Preparation and Application of the New Type Hepatitis E Virus Diagnostic Kit

    • 1. Center for diagnostic Reagents,302nd Hospital of PLA,Bejing 100039

    Abstract: Solid phase enzyme immunoassay (EIA) kit was developed for detecting anti HEV IgG by using synthetic peptide from the open reading frame (ORF)3 and recombinant antigens from ORE2.When the kit was applied to detect HEV antibodies in sera of clinical patients the results were quite consistent with the HEV diagnostic kit from Genlabs.The consistent rate reached 100% (60/60).CV of three lot kit was less than 10%.The kit was stable for 8 months at 4℃or for 4 days at 37℃.The kit was used to detect anti HEV IgG among clinically different patients with acute Non A,Non B,Non C hepatitis,Hepatitis A,Hepatitis B,Hepatitis C and normal human group,the positive rates were 63.2%,13.4%,8.3%,6.6%,2.9%, respectively. The date indicated that the HEV EIA kit is sensitive,specificic and stable,may be used to detect antibody of Hepatitis E Virus among patients and normal human group.

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