Cloning and Sequencing of the Helicoverpa armigera Nuclear Polyhedrosis Virus DNA Polym erase Gene

WANG Gen; ZHANG Chuan-Xi; JI Ping; JIN Wei; WU Xiang-Fu

PDF
Cite
Share

facebook

twitter

google

linkedin

February 1998

WANG Gen, ZHANG Chuan-Xi, JI Ping, JIN Wei and WU Xiang-Fu. Cloning and Sequencing of the Helicoverpa armigera Nuclear Polyhedrosis Virus DNA Polym erase Gene[J]. Virologica Sinica, 1998, 13(1).

Citation: WANG Gen, ZHANG Chuan-Xi, JI Ping, JIN Wei, WU Xiang-Fu. Cloning and Sequencing of the Helicoverpa armigera Nuclear Polyhedrosis Virus DNA Polym erase Gene .VIROLOGICA SINICA, 1998, 13(1) : 82.

中国棉铃虫核型多角体病毒DNA聚合酶基因的克隆和序列分析

1.
中国科学院上海生物化学研究所浙江农业大学蚕学系浙江农业大学应用昆虫学研究所

摘要

应用谷实夜蛾核型多角体病毒（ＨｚＳＮＰＶ）ＤＮＡ聚合酶基因ＨｉｎｄⅢ／ＰｓｔⅠ３５９６ｂｐ片段作探针，经Ｓｏｕｒｔｈｅｒｎｂｌｏｔ杂交，克隆了中国棉铃虫核型多角体病毒（ＨａＳＮＰＶ）完整的ＤＮＡ聚合酶基因，大小约为３．４ｋｂ。限制性内切酶分析表明，ＨａＳＮＰＶＤＮＡ聚合酶基因限制性内切酶图谱与ＨｚＳＮＰＶ相似。用双脱氧链终止法测定该基因部分核苷酸序列（８０５ｂｐ），推导出编码区２０６ａ。序列同源性比较显示，ＨａＳＮＰＶＤＮＡ聚合酶与ＨｚＳＮＰＶ之间具有高度的同源性；与ＬｄＭＮＰＶ、ＡｃＭＮＰＶ、ＢｍＳＮＰＶ、ＣｆＭＮＰＶ和ＯｐＭＮＰＶ也具有一定的同源性.
- 棉铃虫核型多角体病毒 DNA聚合酶基因定位序列复制

Cloning and Sequencing of the Helicoverpa armigera Nuclear Polyhedrosis Virus DNA Polym erase Gene

1.
Shanghai Institute ofBiochevnistry，Academia Sinlca，Shangha i 200031Zhejiang Agricultural University，Hangzhou 310029

Abstract

The location of DNA polymerase gene in the HaSNPV was determined within a 6.6kb HindⅢ J fragment,using a probe prepared from the previously identified DNA polymerase from the Helicoverpa zea single nuclear polyhedrosis virus(HzSNPV).The entire coding region of HaSNPV DNA pol was in a HindⅢ SalI fragment of 3.4kb in size.The restriction enzyme map of HaSNPV DNA pol was similar to that of HzSNPV. A 806bp fragment in the subclone was sequenced and the partial ORF containing 206aa was predicated. Compared with other six members of Baculoviridae ,the DNA polymerase of HaSNPV was highly homologous with that of HzSNPV,and it also showed certain similarity to those of LdMNPV,AcMNPV,BmNPV,CfMNPV and OpMNPV.This study can supply necessity for further attachment of the replication mechanism of HaSNPV.
- Helicoverpa armigera nuclear plyhedrosis virus （HaSNPV） DNA polymerase Gene localization Sequence Replication

References
Proportional views

PDF

Article Metrics

Article views(4134) PDF downloads(828) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequencing of the Helicoverpa armigera Nuclear Polyhedrosis Virus DNA Polym erase Gene

1. Shanghai Institute ofBiochevnistry，Academia Sinlca，Shangha i 200031Zhejiang Agricultural University，Hangzhou 310029

Abstract: The location of DNA polymerase gene in the HaSNPV was determined within a 6.6kb HindⅢ J fragment,using a probe prepared from the previously identified DNA polymerase from the Helicoverpa zea single nuclear polyhedrosis virus(HzSNPV).The entire coding region of HaSNPV DNA pol was in a HindⅢ SalI fragment of 3.4kb in size.The restriction enzyme map of HaSNPV DNA pol was similar to that of HzSNPV. A 806bp fragment in the subclone was sequenced and the partial ORF containing 206aa was predicated. Compared with other six members of Baculoviridae ,the DNA polymerase of HaSNPV was highly homologous with that of HzSNPV,and it also showed certain similarity to those of LdMNPV,AcMNPV,BmNPV,CfMNPV and OpMNPV.This study can supply necessity for further attachment of the replication mechanism of HaSNPV.