LEI Juan-Li, CHEN Jiong, CHEN Jian-Ping, ZHENG Tao and CHENG Ye. Identification of Chinese Fungal·transm itted FilamentousW heat M osaic Virus by RT·PCR and SSCP[J]. Virologica Sinica, 1998, 13(1).
Citation: LEI Juan-Li, CHEN Jiong, CHEN Jian-Ping, ZHENG Tao, CHENG Ye. Identification of Chinese Fungal·transm itted Filamentous W heat M osaic Virus by RT·PCR and SSCP .VIROLOGICA SINICA, 1998, 13(1) : 96.

我国真菌传线状小麦花叶病毒病病原初步鉴定为小麦黄花叶病毒(WYMV)

  • 由禾谷多粘菌(Polymyxagraminis)传播的线状小麦花叶病毒有两种,一种是加拿大首先报道的小麦梭条斑花叶病毒(WSSMV),另一种是日本报道的小麦黄花叶病毒(WYMV)。这两种病毒粒子形态以及血清学性质非常相似,但核酸序列存在一定差异。经反转录聚合酶链反应(RTPCR)和单链构象多态性分析(SSCP),明确我国广泛发生的禾谷多粘菌传线状小麦花叶病毒都是小麦黄花叶病毒,但供试的8个分离物RNA1和RNA2序列存在差异,无一彼此完全相同

Identification of Chinese Fungal·transm itted Filamentous W heat M osaic Virus by RT·PCR and SSCP

  • There are two filamentous wheat mosaic viruses transimitted by the plasmodiophoraceous fungus Polymyxa graminis :one is wheat spindle streak mosaic virus (WSSMV)first reported in Canada and the other is wheat yellow mosaic virus (WYMV) first reported in Japan .These viruses are very similar in particle morphology,biological and serological properties, but different in genomic sequences. The results of RT-PCR and single strand conformation polymorphism analysis (SSCP) indicated that the fungal transmitted filamentous wheat mosaic viral pathogen occurred in successive crops at different sites in China is wheat yellow mosaic virus. SSCP analysis with the fragments amplified using several primer sets all gave different patterns ,indicating the presence of sequence variations among eight isolates collected at different sites in China.

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    Identification of Chinese Fungal·transm itted Filamentous W heat M osaic Virus by RT·PCR and SSCP

    • 1. virology Laboratory ,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021

    Abstract: There are two filamentous wheat mosaic viruses transimitted by the plasmodiophoraceous fungus Polymyxa graminis :one is wheat spindle streak mosaic virus (WSSMV)first reported in Canada and the other is wheat yellow mosaic virus (WYMV) first reported in Japan .These viruses are very similar in particle morphology,biological and serological properties, but different in genomic sequences. The results of RT-PCR and single strand conformation polymorphism analysis (SSCP) indicated that the fungal transmitted filamentous wheat mosaic viral pathogen occurred in successive crops at different sites in China is wheat yellow mosaic virus. SSCP analysis with the fragments amplified using several primer sets all gave different patterns ,indicating the presence of sequence variations among eight isolates collected at different sites in China.

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