Cloning of human interleukin 18 gene and its expression in E. coli

XU Dong-Gang; NIU Jian-Zhang; MENG Zong-Da; DAI Hao-Ying; CHEN Chu-Fen; CUI Ze; SHU Hui-Bin; MENG Wen-Hua

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April 1998

XU Dong-Gang, NIU Jian-Zhang, MENG Zong-Da, DAI Hao-Ying, CHEN Chu-Fen, CUI Ze, SHU Hui-Bin and MENG Wen-Hua. Cloning of human interleukin 18 gene and its expression in E. coli[J]. Virologica Sinica, 1998, 13(2).

Citation: XU Dong-Gang, NIU Jian-Zhang, MENG Zong-Da, DAI Hao-Ying, CHEN Chu-Fen, CUI Ze, SHU Hui-Bin, MENG Wen-Hua. Cloning of human interleukin 18 gene and its expression in E. coli .VIROLOGICA SINICA, 1998, 13(2) : 138.

人白细胞介素-18基因的克隆及其在大肠杆菌中的表达

1.
军事医学科学院基础医学研究所河北省卫生防疫站

摘要

利用反转录ＰＣＲ（ＲＴＰＣＲ）法从人肝组织中分离人白介素１８（ｈＩＬ１８）基因。克隆到Ｔｖｅｃｔｏｒｅａｓｙ载体中，经酶切初步鉴定后进行ＤＮＡ序列分析，结果表明与文献报道完全一致。以ｐＢＶ２２０为表达载体，在大肠杆菌中高效表达了ｈＩＬ１８基因，重组蛋白占菌体总蛋白的２７．２％。为进一步研究其生物活性奠定了重要基础。
- 人白介素-18 cDNA克隆表达大肠杆菌

Cloning of human interleukin 18 gene and its expression in E. coli

1.
Institute of Basic Medical Sciences，Academy of Military Medical Scieces．Beijing 100850 Hebei Provincial Hygiene and Anti-epidemic Station，Baoding 071D00

Abstract

Human interleukin 18 gene was isolated from human liver tissue by reverse transcription polymerase chain reaction.hIL 18 gene was cloned into plasmid pGEM T(Easy)and pBV220.The gene was sequenced and showed no difference with that reported and expresed in E.coli in the form of inclusion bodies.The recombinant protein amounts to 27.2% of total bacterial protein.
- Human interlukin 18 cDNA cloning Gene expression E.coli

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning of human interleukin 18 gene and its expression in E. coli

1. Institute of Basic Medical Sciences，Academy of Military Medical Scieces．Beijing 100850 Hebei Provincial Hygiene and Anti-epidemic Station，Baoding 071D00

Abstract: Human interleukin 18 gene was isolated from human liver tissue by reverse transcription polymerase chain reaction.hIL 18 gene was cloned into plasmid pGEM T(Easy)and pBV220.The gene was sequenced and showed no difference with that reported and expresed in E.coli in the form of inclusion bodies.The recombinant protein amounts to 27.2% of total bacterial protein.