Localization and Cloning of the Chitinase Gene of Heliothis armigera
Single Nuclocapsid Nucleopolyhedrovirus

彭辉银 李星 张双民 Ｂａｓｉｌ Ｍ．Ａｒｉｆ 陈新文 胡志红

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April 1998

彭辉银李星张双民ＢａｓｉｌＭ．Ａｒｉｆ陈新文胡志红, Localization and Cloning of the Chitinase Gene of Heliothis armigeraSingle Nuclocapsid Nucleopolyhedrovirus[J]. Virologica Sinica, 1998, 13(2).

Citation: 彭辉银李星张双民ＢａｓｉｌＭ．Ａｒｉｆ陈新文胡志红, . Localization and Cloning of the Chitinase Gene of Heliothis armigera Single Nuclocapsid Nucleopolyhedrovirus .VIROLOGICA SINICA, 1998, 13(2) : 139.

中国棉铃虫核型多角体病毒几丁质酶基因的定位与克隆

1.
中国科学院武汉病毒研究所

摘要

以α３２ＰｄＡＴＰ标记含ＣｆＭＮＰＶ几丁质酶基因的重组质粒为探针，在６８℃条件下对棉铃虫单粒包埋核型多角体病毒（ＨａＳＮＰＶ）进行Ｓｏｕｔｈｅｒｎ杂交，将ＨａＳＮＰＶ的几丁质酶基因分别定位在ＢａｍＨＩＥ、ＢｇｌⅡＥ、ＥｃｏＲＩＧ、ＨｉｎｄⅢＦ、ＸｂａＩＨ、ＢａｍＨＩ＋ＨｉｎｄＩＩＭ和ＢａｍＨＩ＋ＸｂａＩＨ，并以ｐＴＺ１９Ｒ为载体获得了ＸｂａＩＨ片段克隆。
- 棉铃虫核型多角体病毒几丁质酶基因 Southern杂交基因定位

Localization and Cloning of the Chitinase Gene of Heliothis armigera Single Nuclocapsid Nucleopolyhedrovirus

1.
Wuhan Institute of Virology，Chinese Academy Sciences．Wuhan 430071Forest Pest Management Institute，Sault Ste Marie．Canada，P6A5M7

Abstract

The chitinase gene of Heliothis armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV) was localized on BamHI E, BglII E,EcoRI G,HindIII F,XbaI H,BamHI+HindIII M,and BamHI+XbaI H fragments, using the α P 32 dATP labelled recombinant plasmid harboring Choristoneura fumiferana multiple nucleocapsid nucleopolyhedrovirus (CfMNPV)DNA HindIII M which contains chitinase gene as the probe. The XbaI H fragment of HaSNPV was cloned into pTZ 19R vector for further sequencing the chitinase gene.
- Heliothis armigera single nucleocapsid nucleopolyhedrovirus（HaSNPV） Chitinase gene Southern hybridization Gene localization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Localization and Cloning of the Chitinase Gene of Heliothis armigera Single Nuclocapsid Nucleopolyhedrovirus

1. Wuhan Institute of Virology，Chinese Academy Sciences．Wuhan 430071Forest Pest Management Institute，Sault Ste Marie．Canada，P6A5M7

Abstract: The chitinase gene of Heliothis armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV) was localized on BamHI E, BglII E,EcoRI G,HindIII F,XbaI H,BamHI+HindIII M,and BamHI+XbaI H fragments, using the α P 32 dATP labelled recombinant plasmid harboring Choristoneura fumiferana multiple nucleocapsid nucleopolyhedrovirus (CfMNPV)DNA HindIII M which contains chitinase gene as the probe. The XbaI H fragment of HaSNPV was cloned into pTZ 19R vector for further sequencing the chitinase gene.