LIU Ming-Jun, WANG Zheng-Dang and WANG Sheng-Qi. Detection of the Group B Rotavirus with the Probe0f 0lig0nucle0tides Labelled with Biotin[J]. Virologica Sinica, 1998, 13(2).
Citation: LIU Ming-Jun, WANG Zheng-Dang, WANG Sheng-Qi. Detection of the Group B Rotavirus with the Probe 0f 0lig0nucle0tides Labelled with Biotin .VIROLOGICA SINICA, 1998, 13(2) : 159.

生物素标记寡核苷酸探针检测B群轮状病毒

  • 用5′端接氨基标记法,用生物素对B群轮状病毒(GBRV)IDIR株具有群特异性的3片段基因上23bp寡核苷酸序列进行标记,经高效薄层层析板(HPTLC)纯化,制备了B群轮状病毒生物素寡核苷酸探针,探针显色灵敏度达10Pg,与GBRV、KB63株基因杂交可检测到100pg靶序列,而与A群和C群轮状病毒及无关基因均不产生杂交信号,该探针可用于B群轮状病毒的检测、鉴定,以及同群不同毒株间基因相关性研究。

Detection of the Group B Rotavirus with the Probe 0f 0lig0nucle0tides Labelled with Biotin

  • According to segment 3 sequence of group B rotavirus (GBRV)IDIR strain the 23 bp oligonucleotide was synthesized and labelled with biotin.The probe was purified by HPTLC.Its sensitivity is up to 10pg by a colorimetric reaction.The probe can detect at least 100pg target seuqence of GBRV and does not hybridize to Group A and Group C rotavirus.The results showed that the probe has high specificity and sensitivity.It can be used to detect and identify GBRV,and also to research the genetic relativity of GBRV gene mutation and rearrangement.

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    Detection of the Group B Rotavirus with the Probe 0f 0lig0nucle0tides Labelled with Biotin

    • 1. Animal Biotechnology Laboratory,Xinjiang Academy of Animal Science,Urumqi 830000

    Abstract: According to segment 3 sequence of group B rotavirus (GBRV)IDIR strain the 23 bp oligonucleotide was synthesized and labelled with biotin.The probe was purified by HPTLC.Its sensitivity is up to 10pg by a colorimetric reaction.The probe can detect at least 100pg target seuqence of GBRV and does not hybridize to Group A and Group C rotavirus.The results showed that the probe has high specificity and sensitivity.It can be used to detect and identify GBRV,and also to research the genetic relativity of GBRV gene mutation and rearrangement.

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