Cloning and Sequence Characterization of L4 Nonstructural
100K Protein Gene of Egg Drop Syndrome Virus

LI Mao-Xiang; JIN Qi; ZHANG Jin-Gang; CENG Li-Yu; TAO Er-Mei; YAN Shen; HOU Yun-De

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April 1998

LI Mao-Xiang, JIN Qi, ZHANG Jin-Gang, CENG Li-Yu, TAO Er-Mei, YAN Shen and HOU Yun-De. Cloning and Sequence Characterization of L4 Nonstructural100K Protein Gene of Egg Drop Syndrome Virus[J]. Virologica Sinica, 1998, 13(2).

Citation: LI Mao-Xiang, JIN Qi, ZHANG Jin-Gang, CENG Li-Yu, TAO Er-Mei, YAN Shen, HOU Yun-De. Cloning and Sequence Characterization of L4 Nonstructural 100K Protein Gene of Egg Drop Syndrome Virus .VIROLOGICA SINICA, 1998, 13(2) : 160.

减蛋综合征病毒100K蛋白基因的克隆与序列分析

1.
中国预防医学科学院病毒基因工程国家重点实验室中国人民解放军长春农牧大学军事兽医研究所

摘要

用常规方法提取减蛋综合征病毒（ＥＤＳＶ）中国分离株（ＡＡ２株）病毒ＤＮＡ，分别构建了限制性内切酶ＨｉｎｄⅢ、ＳｐｈⅠ、ＰｓｔⅠ水解片段的全基因文库，并对其中１００Ｋ蛋白基因的序列进行了分析。ＥＤＳＶ１００Ｋ蛋白基因位于减蛋综合征病毒基因组５５．７～６４．８物理图谱单位（ｍ．ｕ），共２０９１个核苷酸（ｎｔ），其编码产物由６９６个氨基酸（ａａ）组成，推测其分子量为７７．７ｋＤ。编码蛋白氨基酸同源性分析表明，ＥＤＳＶ１００Ｋ蛋白与人腺病毒（Ａｄ２、Ａｄ５、Ａｄ１２、Ａｄ４１）、Ⅰ群禽腺病毒（ＣＥＬＯ和ＦＡＶ１０）的同源性为３２．３～３４．４％之间，而与羊腺病毒（ＯＡＶ）的同源性达到５６．４％。
- 减蛋综合征病毒 DNA序列分析 100K蛋白

Cloning and Sequence Characterization of L4 Nonstructural 100K Protein Gene of Egg Drop Syndrome Virus

1.
State Key La&aratory ofMolez-ula~r Virology and Genetic Engineering，Belting 100052The Military Veterinary Institute,University of Agricuture and Animal Science．Changchun 1319062

Abstract

The nucleotide sequence and location of the L4 nonstructural 100 K protein gene of the egg drop syndrome virus (EDSV),a strain AA 2 previously isolated from China,were determined.The 100K protein gene located at 55.7-64.8 m.u.has a length of 2091 nt and codes for a polypeptide of 696 amino acids (aa)with a molecular weight of 77.7kD. Comparison of the amino acid sequence of the 100K proteins from human adenoviruses and fowl adenoviruses of group I revealed a homology from 32.3% to 34.4%.Remarkably,EDSV 100K protein shares high homology (56.4% on amino acid level)with that of ovine adenovirus.
- Egg drop syndrome virus DNA sequencing 100K protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequence Characterization of L4 Nonstructural 100K Protein Gene of Egg Drop Syndrome Virus

1. State Key La&aratory ofMolez-ula~r Virology and Genetic Engineering，Belting 100052The Military Veterinary Institute,University of Agricuture and Animal Science．Changchun 1319062

Abstract: The nucleotide sequence and location of the L4 nonstructural 100 K protein gene of the egg drop syndrome virus (EDSV),a strain AA 2 previously isolated from China,were determined.The 100K protein gene located at 55.7-64.8 m.u.has a length of 2091 nt and codes for a polypeptide of 696 amino acids (aa)with a molecular weight of 77.7kD. Comparison of the amino acid sequence of the 100K proteins from human adenoviruses and fowl adenoviruses of group I revealed a homology from 32.3% to 34.4%.Remarkably,EDSV 100K protein shares high homology (56.4% on amino acid level)with that of ovine adenovirus.