Sequencing of a cDNA fragment of NS5A region of the defective hepatitis C
virus

SHU Fen-Lu; DAI Hao-Ying; MENG Qiang-Hua; QI Zhong-Tian

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April 1998

SHU Fen-Lu, DAI Hao-Ying, MENG Qiang-Hua and QI Zhong-Tian. Sequencing of a cDNA fragment of NS5A region of the defective hepatitis Cvirus[J]. Virologica Sinica, 1998, 13(2).

Citation: SHU Fen-Lu, DAI Hao-Ying, MENG Qiang-Hua, QI Zhong-Tian. Sequencing of a cDNA fragment of NS5A region of the defective hepatitis C virus .VIROLOGICA SINICA, 1998, 13(2) : 182.

一株丙型肝炎病毒缺陷株NS5A基因片段的序列分析

1.
第二军医大学微生物学教研室军事医学科学院基础医学研究所北京佑安医院

摘要

Ｑ丙型肝炎是由丙型肝炎病毒（ＨＣＶ）引起的一种严重的传染病。丙型肝炎病毒主要通过输血和应用不洁的血液制品传播，所以利用敏感、特异的检测方法筛选献血员对丙型肝炎的预防尤为重要。现在第二代ＨＣＶ检测试剂已大批应用，加入ＮＳ５Ａ抗原的第三代试剂也正在研制中...
- 丙型肝炎病毒缺陷株序列分析

Sequencing of a cDNA fragment of NS5A region of the defective hepatitis C virus

1.
Department of Microbiology,Second Military Medical University．Shanghai 200433

Abstract

Sensitive and specific anti HCV diagnostic kits are very important for preventing of the transmission of hepatitis C virus. Adding HCV NS5A antigen in diagnostic kits may increase the sensitivity of diagnostic reagents. The result of sequencing of NS5A cDNA fragment showed that it shares over 90% nucleotide acid and amino acid homology with HCV BK etc., so the isolate of this study belongs to HCV II. Besides, a mutation at 411nt in NS5A segment, which created a stop codon TGA in the reading frame was found, indicating that the isolate might be a defective virus. Perhaps the defective virus causes the persistent infection of HCV.
- Hepatitis C virus Defective virus Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequencing of a cDNA fragment of NS5A region of the defective hepatitis C virus

1. Department of Microbiology,Second Military Medical University．Shanghai 200433

Abstract: Sensitive and specific anti HCV diagnostic kits are very important for preventing of the transmission of hepatitis C virus. Adding HCV NS5A antigen in diagnostic kits may increase the sensitivity of diagnostic reagents. The result of sequencing of NS5A cDNA fragment showed that it shares over 90% nucleotide acid and amino acid homology with HCV BK etc., so the isolate of this study belongs to HCV II. Besides, a mutation at 411nt in NS5A segment, which created a stop codon TGA in the reading frame was found, indicating that the isolate might be a defective virus. Perhaps the defective virus causes the persistent infection of HCV.