HAN Xin-Bing, ZHANG Fu-Ping, WANG Zheng-Dang and HUANG Jia-Si. Detection of group B rotavirus by RT-PCR[J]. Virologica Sinica, 1998, 13(3).
Citation: HAN Xin-Bing, ZHANG Fu-Ping, WANG Zheng-Dang, HUANG Jia-Si. Detection of group B rotavirus by RT-PCR .VIROLOGICA SINICA, 1998, 13(3) : 279.

用反转录-聚合酶链反应检测B组轮状病毒

  • 轮状病毒(Rotavirus)是属于呼肠病毒科(Reoviridae)的双链RNA(dsRNA)病毒。至今已将轮状病毒分为七个组(A~G)。已经发现的B组轮状病毒分别来自人、大鼠、牛、猪、羊。近十年来,通过轮状病毒的研究,轮状病毒B组已被公认为引起人...

Detection of group B rotavirus by RT-PCR

  • A diagnostic assay was presented to detect group B rotavirus (GBRV) in fecal specimens with reverse transcription polymerase chain reaction (RT PCR). GBRV double strand RNAs (dsRNA) isolated from stool samples were reverse transcribed and amplified by PCR using two oligonucleotide primers which were derived from genomic segment 3 of the IDIR (intestinal disease of infants rat) strain of GBRV. This RT PCR assay permitted the sensitive and specific detection of a variety of GBRV in fecal specimens. Wild GBRV strains of lamb, kid were detected with these primer pairs by RT PCR. Moreover, RT PCR also permitted the detection of genomic RNA of lamb GBRV KB 63 strain which has been adapted to serial passage in cattle. The specific PCR products of 290 bp suggested that GBRV from lamb, kid and calf had identical sequences in genomic RNA;

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    Detection of group B rotavirus by RT-PCR

    • 1. Faculty of Animal Medicine.Xinjiang Agricultural University.Urumqi,Xinjiang 830052 State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100080

    Abstract: A diagnostic assay was presented to detect group B rotavirus (GBRV) in fecal specimens with reverse transcription polymerase chain reaction (RT PCR). GBRV double strand RNAs (dsRNA) isolated from stool samples were reverse transcribed and amplified by PCR using two oligonucleotide primers which were derived from genomic segment 3 of the IDIR (intestinal disease of infants rat) strain of GBRV. This RT PCR assay permitted the sensitive and specific detection of a variety of GBRV in fecal specimens. Wild GBRV strains of lamb, kid were detected with these primer pairs by RT PCR. Moreover, RT PCR also permitted the detection of genomic RNA of lamb GBRV KB 63 strain which has been adapted to serial passage in cattle. The specific PCR products of 290 bp suggested that GBRV from lamb, kid and calf had identical sequences in genomic RNA;

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