ZHENG Guan-Ceng, XIE Dong-Gui and SHU Zhi-Yong. Isolation, Identification and Biological Propertiesof Ten-Strain Variclla-Zoster Viruses[J]. Virologica Sinica, 1999, 14(1): 11-16.
Citation: ZHENG Guan-Ceng, XIE Dong-Gui, SHU Zhi-Yong. Isolation, Identification and Biological Propertiesof Ten-Strain Variclla-Zoster Viruses .VIROLOGICA SINICA, 1999, 14(1) : 11-16.

10株水痘带状疱疹病毒的分离鉴定与生物学特性

  • 用Veto E6细胞从12倒水痘及带状疱疹病^水疱液中分离到l0株病毒,分离阳性率为83 3%。10株病毒均具有使感染细胞圆缩、融合、脱落等典型的vzv 局灶性细胞病变(CPE)特点,CPE随着传代次数增加而加快 用带状疤疹恢复期病^血清作闻接免疫荧光染色镜检,可见典型的感染细胞核内荧光块。1O株病毒感染细胞制成的抗原片.检测5倒带状疱疹病^急性期及恢复期血清。荧光抗体滴度均有4倍以上增高。Vzv对Vero细胞敏感;对沙鼠肾,胎兔肾.胎豚鼠肾、肺、脾原代单层细胞不敏感;对乳小白鼠不敏感。毒种在一20℃保存一周内死亡;但在30%脱脂牛奶、20%小牛血清及10% 山梨醇Eagles液中,蔽体或真空冷冻干燥一100℃可保存二年以上。

Isolation, Identification and Biological Propertiesof Ten-Strain Variclla-Zoster Viruses

  • Ten strains of virus were isolated from vesicle fluid of skin lesions of 12 patients with variclla or herpes zoster by means of Vem-E6 eeUs.The postive rate was 83.3% These viruses all cause typical cell damages such as rounded and shrivelled,fusion and fallout.These damages got quick following increased future degenerations The typical fluorescence lump of nucleus within the infected cells would be found by indirect immunofluorescence technique.The antigen samples prepared with virusinfected cells could be used to examine the sera of patients with herpes zoster and it could be found that the antibody titer in recovering stage was four times as much as that in acute stage.VZV was sensitive to Vero cells and not sensitive to the primary simple-lager cells from M eriones unguiculatus kidney, fetal rabbit kidney and fetal guinea pig kidney,lung and spleen VZV was nsensitive to newborn white mouse too.The viruses would die within one week at 一20℃ 。but they can be preserved in Eagle。s solution containing de-fat milk,newborn bovine serum and sorbitol at 一100℃ over two years

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    Isolation, Identification and Biological Propertiesof Ten-Strain Variclla-Zoster Viruses

    • 1. Taizhou Ciey Sanitary and Epide~nic Prevention Stalion,Taizhou,Zhejiang Province 317000

    Abstract: Ten strains of virus were isolated from vesicle fluid of skin lesions of 12 patients with variclla or herpes zoster by means of Vem-E6 eeUs.The postive rate was 83.3% These viruses all cause typical cell damages such as rounded and shrivelled,fusion and fallout.These damages got quick following increased future degenerations The typical fluorescence lump of nucleus within the infected cells would be found by indirect immunofluorescence technique.The antigen samples prepared with virusinfected cells could be used to examine the sera of patients with herpes zoster and it could be found that the antibody titer in recovering stage was four times as much as that in acute stage.VZV was sensitive to Vero cells and not sensitive to the primary simple-lager cells from M eriones unguiculatus kidney, fetal rabbit kidney and fetal guinea pig kidney,lung and spleen VZV was nsensitive to newborn white mouse too.The viruses would die within one week at 一20℃ 。but they can be preserved in Eagle。s solution containing de-fat milk,newborn bovine serum and sorbitol at 一100℃ over two years

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