Genotyping of HIV-1 in Anti-HIV-1 Sero-Positive Plasma

ZHONG Beng; LIU Gui-Zhen; SHU Wei; DUAN Ji-Liang; ZHANG Yi-Huang

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February 1999

ZHONG Beng, LIU Gui-Zhen, SHU Wei, DUAN Ji-Liang and ZHANG Yi-Huang. Genotyping of HIV-1 in Anti-HIV-1 Sero-Positive Plasma[J]. Virologica Sinica, 1999, 14(1): 17-22.

Citation: ZHONG Beng, LIU Gui-Zhen, SHU Wei, DUAN Ji-Liang, ZHANG Yi-Huang. Genotyping of HIV-1 in Anti-HIV-1 Sero-Positive Plasma .VIROLOGICA SINICA, 1999, 14(1) : 17-22.

HIV-1抗体阳性血浆中HIV1病毒基因分型研究

1.
卫生部上海生物制品研究所

摘要

为了解ＨＩＶ抗体阳性血浆中的ＨＩＶ１病毒基因亚型的情况，应用逆转录ＰＣＲ和ＤＮＡ序列测定技术，对６份获自高危人群的抗ＨＩＶ１阳性血浆进行序列分析和基因亚型分型的研究，结果表明均属ＨＩＶ１Ｂ亚型。Ｖ３环氨基酸序列分析指出这些ＨＩＶ１Ｂ亚型病毒株与泰国ＨＩＶ１Ｂ亚型病毒株核苷酸和氨基酸序列相似；同时发现ＨＩＶ１ｃＤＮＡ和氨基酸序列均相同，推测这６份标本可能来自同时感染同一株ＨＩＶ病毒的感染者。本研究对了解高危人群中ＨＩＶ１流行的遗传变异和ＨＩＶ１亚型病毒株的分子流行病分析具有一定的意义。
- 人免疫缺陷病毒
- , 逆转录PCR
- , DNA序列测定
- , 基因亚型

Genotyping of HIV-1 in Anti-HIV-1 Sero-Positive Plasma

1.
Shanghai Institute of Biologica[Products，Shanghai 200052

Abstract

Six of anti HIV 1 sero positive plasma derived from a local high risk population were investigated by RT PCR and cDNA sequencing. The results indicated that HIV 1 prevalent in a local high risk population belonged to HIV 1 subtype B. The sequences of nucleic acids and amino acids in V3 loop of 6 cDNA were completely similar to that of Thailand HIV 1 subtype B strains. Besides, the sequences of cDNA and amino acid from 6 plasma samples were identical. It implied that they might be derived from 6 HIV 1 infected people who were infected with HIV 1 at the same period. This study could provide a better understanding of prevelence of HIV 1 subtype strains in a local high risk population.
- HIV 1
- , cDNA sequencing
- , Geno subtype

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Genotyping of HIV-1 in Anti-HIV-1 Sero-Positive Plasma

1. Shanghai Institute of Biologica[Products，Shanghai 200052

Abstract: Six of anti HIV 1 sero positive plasma derived from a local high risk population were investigated by RT PCR and cDNA sequencing. The results indicated that HIV 1 prevalent in a local high risk population belonged to HIV 1 subtype B. The sequences of nucleic acids and amino acids in V3 loop of 6 cDNA were completely similar to that of Thailand HIV 1 subtype B strains. Besides, the sequences of cDNA and amino acid from 6 plasma samples were identical. It implied that they might be derived from 6 HIV 1 infected people who were infected with HIV 1 at the same period. This study could provide a better understanding of prevelence of HIV 1 subtype strains in a local high risk population.