采用ＨＧＶＮＳ５特异的２对引物，对两个香港株和一个广东株ＨＧＶＲＮＡ进行逆转录套式ＰＣＲ扩增，ＰＣＲ产物克隆入ｐＵＣ１９，重组质粒转化ＤＨ５和ＪＭ１０９菌株。ＰＣＲ和酶切法鉴定阳性克隆，双脱氧链末端终止法测定核苷酸序列并进行同源性分析。结果发现核苷酸变异呈散在分布，三株间核苷酸和氨基酸序列同源性分别为９３．３％～９４％及９７％～９９．２％，与已报道的中国株（ＣＮ）相比，则同源性分别为９０％～９１．２％和９４％～９６．３％，与美国株（ＰＮＦ２１６１及Ｒ１０２９１）相比，为８７．１％～８９．５％和９５．２％～９７％，而与西非株（ＧＢＶＣ）相比，则达９１．４％～９３．８％和９７％～９７．９％。提示ＨＧＶＮＳ５区核苷酸和氨基酸序列相对保守，不同ＨＧＶ株存在一定的地区差异。

HGV NS5 cDNA of two Hong Kong strains and one Guangdong strain were amplified by re verse transcription polymerase chain reaction (RT-PCR)．T}le products were inserted into pUC19 vect0rs respectively．~fter transfecting DH5a and JM 109 strains．the reeombinants were screened and identified by PCR and dig ted wlth restriction erldDnudeses．n 1e nudeotldes were sequenced by the Ndmxy chain termination method n 1e homologies d HGV NS5 r~ ion nucleotide and d~umdlTlillO acid sequences were 93 3％ 一94％ and 97％ 一99．2％ re印ecnve【v between eachHangKong strainandtheGtm~dong strain an dwere 90％ 一91 2％ and 94％ 一96 3％ ，87．1％ 一89．5％ an d 95．2％ 一97％ 91 4％ 93 8％ and 97％ 97 9％ respectively Compared with the reported China isolate(CN) American iso lates(PNF2161 and R10291)，and West African lsolated(GBV-C) T}1e variant sites of the nudeotides appear spor~ ieally T}le nucleotide an d ded umd amino acid seQuences of HGV NS5 genornic region are relatively conserved ，andit also sog gests thatthe nudeotide variation ofHGV may beinfluenced by geo graphical factors