Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells

YANG Tie-Qiu; LIU Jian-Jun; XIAO Gong; WEN Li; GUO Chu-Fang; SONG Jie-Ping

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April 1999

YANG Tie-Qiu, LIU Jian-Jun, XIAO Gong, WEN Li, GUO Chu-Fang and SONG Jie-Ping. Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells[J]. Virologica Sinica, 1999, 14(2): 106-109.

Citation: YANG Tie-Qiu, LIU Jian-Jun, XIAO Gong, WEN Li, GUO Chu-Fang, SONG Jie-Ping. Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells .VIROLOGICA SINICA, 1999, 14(2) : 106-109.

柯萨奇B组病毒在Hep-2细胞内增殖特性的研究

1.
湖北医科大学病毒研究所，武汉43007

摘要

采用IFA、RT-PCR法观察了柯萨奇B组病毒(CBV)5型在Hep-2细胞内的增殖动态。CBV感染细胞后4 h可检出病毒RNA，8 h可检出病毒抗原，12 h可观察到细胞病变．20 h可检出病毒颗粒。结果为CBV增殖特性的研究提供了新的资米斗．并指出PCR是监测CBV增殖的敏感方法
- 柯萨奇B组病毒
- , 增殖
- , 聚合酶链反应

Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells

1.
Virus Research Institute，Huhei Medica[University， Wuhan 430071

Abstract

The dynamic change of coxsackie B virus(CBV)5 type propagation was studied in Hep一2 cells by immunofluorescence assay，reverse transcription polymerase chain reaction and cy tological methods The results showed that CBV RNA muld be detected at 4 hours in postinfec tion，vira1 antigen was positive at 8 hours and cytopathic effect could be found at 12 hours The vira1 particles could be observed at 20 hours in postinfeedon These results provided new data for observation of CBV propagation，also，it indicated that the reverse transcription polymerase chain reaction was a sensitive technique for m onitoring CBV propagation
- Coxsackie B virus
- , Propagation
- , Polymerase chain reaction

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells

1. Virus Research Institute，Huhei Medica[University， Wuhan 430071

Abstract: The dynamic change of coxsackie B virus(CBV)5 type propagation was studied in Hep一2 cells by immunofluorescence assay，reverse transcription polymerase chain reaction and cy tological methods The results showed that CBV RNA muld be detected at 4 hours in postinfec tion，vira1 antigen was positive at 8 hours and cytopathic effect could be found at 12 hours The vira1 particles could be observed at 20 hours in postinfeedon These results provided new data for observation of CBV propagation，also，it indicated that the reverse transcription polymerase chain reaction was a sensitive technique for m onitoring CBV propagation