Establishment of Transient Expression System I of Poliovirus Defective Recombinant Vector

LI Qi-Han; DIAO Gong-Ling; WANG Li-Chun; SUN Meng; JIANG Chi; DONG Cheng-Gong; WANG Jiong-

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April 1999

LI Qi-Han, DIAO Gong-Ling, WANG Li-Chun, SUN Meng, JIANG Chi, DONG Cheng-Gong and WANG Jiong-. Establishment of Transient Expression System I of Poliovirus Defective Recombinant Vector[J]. Virologica Sinica, 1999, 14(2): 116-122.

Citation: LI Qi-Han, DIAO Gong-Ling, WANG Li-Chun, SUN Meng, JIANG Chi, DONG Cheng-Gong, WANG Jiong-. Establishment of Transient Expression System I of Poliovirus Defective Recombinant Vector .VIROLOGICA SINICA, 1999, 14(2) : 116-122.

脊髓灰质炎病毒缺陷型载体瞬时表达系统Ⅰ的构建

1.
中国医学科学院中国协和医科大学医学生物研究所，昆明650107

摘要

为探索可表达较大片段外瓶基因的脊灰病毒重组载体．以HBVS基因置换脊灰病毒的P1基因，同时另一途径提供脊灰病毒P1结构蛋白，经转染八Hela细胞中形成映陷型重组病毒颗粒，此病毒可感染新的细胞，并表达其重组的外源基因．但不产生于代病毒实验结果表明，这种瞬时表达系统的构建，为脊灰病毒缺陷型重组载体用于基因转导技术打下基础。
- 脊灰病毒
- , 映陷型重组体
- , 外源基因

Establishment of Transient Expression System I of Poliovirus Defective Recombinant Vector

1.
Insliaute ofMedical Bidogy，CAMS．PUMC Kunming 650107

Abstract

For investigating poliovirus recombinant defective vector system, the separated genes of poliovirus structure protein and replicase were induced into cell, the defective recombinant virus could be assembled. This virus is able to infect the cell and to express the extraneous gene in the cell, and, no filial virus would be produced. The transient expression system has been established in this work for investigating the applied technique of poliovirus defective recombinant system in gene transduction.
- Poliovirus
- , Defective recombinant
- , Extraneous gene

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Establishment of Transient Expression System I of Poliovirus Defective Recombinant Vector

1. Insliaute ofMedical Bidogy，CAMS．PUMC Kunming 650107

Abstract: For investigating poliovirus recombinant defective vector system, the separated genes of poliovirus structure protein and replicase were induced into cell, the defective recombinant virus could be assembled. This virus is able to infect the cell and to express the extraneous gene in the cell, and, no filial virus would be produced. The transient expression system has been established in this work for investigating the applied technique of poliovirus defective recombinant system in gene transduction.