Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus

ZHANG Xiao-Dong; ZHANG Jia-Min; GUO Hai-Chao; SHU Li-Hua; HU Yuan-Yang

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April 1999

ZHANG Xiao-Dong, ZHANG Jia-Min, GUO Hai-Chao, SHU Li-Hua and HU Yuan-Yang. Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus[J]. Virologica Sinica, 1999, 14(2): 152-156.

Citation: ZHANG Xiao-Dong, ZHANG Jia-Min, GUO Hai-Chao, SHU Li-Hua, HU Yuan-Yang. Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus .VIROLOGICA SINICA, 1999, 14(2) : 152-156.

重组质粒转染蟑螂对黑胸大蠊浓核病毒的拯救

1.
武泣大学病毒研究所，武汉430072

摘要

用Ｇｌａｓｓｍｉｌｋ法纯化ＰｆＤＮＶｄｓＤＮＡ，在其３端和ＰｓｔⅠ切开的ｐＵＣ１１９质粒的３端分别加ｄＧ和ｄＣ尾，连接、转化、筛选得到分子量为８．７ｋｂ的重组质粒。通过酶切证明插入ＤＮＡ为ＰｆＤＮＶ全基因组ＤＮＡ。利用ＤＥＡＥｄｅｘｔｒａｎ转染技术，将此重组质粒导入黑胸大蠊幼虫体内，此重组质粒能使虫体发病死亡。电镜超薄切片观察发现虫体细胞内存在大量的病毒粒子。同样，在免疫扩散实验中虫体ＰＢＳ浸出液能与抗ＰｆＤＮＶ的抗体产生沉淀线。用重组质粒感染致死的虫体喂食健康黑胸大蠊，也能使其发病死亡，通过电镜可以观察到在细胞内增殖的病毒粒子。
- 浓核病毒
- , 黑胸大蠊
- , 重组质粒
- , 病毒拯救

Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus

1.
1nsitute Virology，Wuhan University，Wuhan 430072

Abstract

An infectious genome of the Periplanta fuliginosa densovirus (PfDNV) has been cloned into the bacterial plasmid pUC119. The viral genome could be rescued from the recombinant plasmid PfNDV pUC119 by transfection to sensitive Periplanta fuliginosa larvae. Virions can be found in the cells of the transfected larvaes by electron microscope, and the rescued virions were as infectious as wild type virions. In contrast, the viral genome could not be rescued from the recombinant plasmid deleted one extremity.
- Densovirus
- , Periplanta^ liginosa
- , ReCOmbinant plasmid
- , Rescue

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus

1. 1nsitute Virology，Wuhan University，Wuhan 430072

Abstract: An infectious genome of the Periplanta fuliginosa densovirus (PfDNV) has been cloned into the bacterial plasmid pUC119. The viral genome could be rescued from the recombinant plasmid PfNDV pUC119 by transfection to sensitive Periplanta fuliginosa larvae. Virions can be found in the cells of the transfected larvaes by electron microscope, and the rescued virions were as infectious as wild type virions. In contrast, the viral genome could not be rescued from the recombinant plasmid deleted one extremity.