WANG Zhen-Dong, ZHANG Min and SHANG Tian-Yi-Lang. The Expression of Potato Virus Y Common Strain(PVY0)Coat Protein Gene in Escherichia coli[J]. Virologica Sinica, 1999, 14(2): 157-162.
Citation:
WANG Zhen-Dong, ZHANG Min, SHANG Tian-Yi-Lang.
The Expression of Potato Virus Y Common Strain(PVY0)Coat Protein Gene in Escherichia coli .VIROLOGICA SINICA, 1999, 14(2)
: 157-162.
-
摘要
将马铃薯Y病毒普通系(PVY0)的外壳蛋白基因克隆到表达质粒pMALc2中,构建这一基因在大肠杆菌中的表达载体pMALc2PVY0CP。SDSPAGE及Westernbloting检测结果表明,这一表达栽体在E.coliDH5中经IPTG诱导可表达分子量为71.8kDa的特异性融合蛋白。以amyloseresin亲合柱层析纯化这一融合蛋白为抗原,免疫家兔制备了效价为1∶1024的特异性抗血清。用该抗血清可通过对流免疫电泳、免疫双扩散及Westernbloting对PVY进行检测
The Expression of Potato Virus Y Common Strain(PVY0)Coat Protein Gene in Escherichia coli