Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells[J]. Virologica Sinica, 1999, 14(2): 181-184.
Citation: . Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells .VIROLOGICA SINICA, 1999, 14(2) : 181-184.

汉滩病毒S片段基因编码区在Vero-E6细胞中的表达

  • 汉滩词病 毒倡于布尼亚病毒芈科汉坦精毒属, 主要引起人类肾综合征出血热? 。我国是肾综合征出血热发生和流行的主要疫区,该病起病急、病死率高,流行范围广,因此寻找安全有效、低廉的疫苗是控制本病流行和发生的关键。基因免疫是近年来微生物学和免疫学研究的新热点_2 J,也是达到上述目的的一个新途径。它将某病原体的抗原基因插入到真核表达载体中.构成含有病原体抗原基因的重组质粒,再用该质粒免疫动物。由于基因免疫具有同时激发机体的体液免疫和细胞免疫的特点,而且操作简单,成本低廉,因此探索基因免疫预防肾综合征出血热具有现实意义。我们将汉滩病毒s片段编码区基因克隆至真核表达载体质粒pVR1012的多克隆位点上,构建了pVRs22重组表达质粒.并进行了体外非洲绿猴肾细胞Vero- 的瞬时转染和表达,为汉滩病毒S基因的动物免疫奠定了基础。现将结果报告如下。

Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells

  • The coding region of S genorae segment of Hantaan virus(76/118 strain)was inserted into the eukarytic expression plasmld pVR1012 The recombinant expression plasmid pVRS22 was c。nstructed.Ver0一E cells were transiently tmnsfected in vitro with pVRS22 plasmid.The transient expresslon of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indi rect immunofluorescence asay(IFA)with monoclonal antibody 5H5 against Hantaan virus

  • 加载中
  • 加载中

Article Metrics

Article views(3692) PDF downloads(820) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells

    • 1. Tangdu Hospital,The Fourth Military Medical University,Xi'an 710038

    Abstract: The coding region of S genorae segment of Hantaan virus(76/118 strain)was inserted into the eukarytic expression plasmld pVR1012 The recombinant expression plasmid pVRS22 was c。nstructed.Ver0一E cells were transiently tmnsfected in vitro with pVRS22 plasmid.The transient expresslon of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indi rect immunofluorescence asay(IFA)with monoclonal antibody 5H5 against Hantaan virus

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return