应用差别ｐＨ值沉淀蛋白质的原理，建立了水稻条纹病毒病特异蛋白（ＳＰ）的两种提纯方法。这两种方法都可以从病叶中提纯到大量的ＳＰ，其粗提纯量分别为０．８和２．０ｍｇ／ｇ病叶。通过ＳＤＳＰＡＧＥ分离后得到了精提纯的蛋白，其分子量为２０．１ｋＤａ。将粗提纯和精提纯的ＳＰ分别免疫兔子，制备出效价为５１２００和６４００的抗血清。将效价为６４００的高度特异性的抗血清用于研究ＲＳＶＳＰ与ＲＳＶＣＰ及同属的水稻草状矮化病毒（ＲＧＳＶ）ＳＰ、ＣＰ之间的血清学关系，结果表明，ＲＳＶＳＰ的抗血清与ＲＧＳＶＣＰ、ＲＳＶＣＰ之间无反应，但可与ＲＧＳＶＳＰ微弱反应；而ＲＧＳＶＳＰ、ＣＰ及ＲＳＶＣＰ的抗血清与ＲＳＶＳＰ之间都无血清学反应。结果证实了ＲＳＶ和ＲＧＳＶ之间存在着进化上的亲缘关系

According to the differential pH precipitation of proteins, two methods were established for purification of disease specific protein (SP) in rice plants infected by rice stripe virus (RSV), a member of \%Tenuivirus.\% The yields obtained by the two methods were 0.8 and 2.0 mg SP per g of infected tissues, respectively. After separation by SDS PAGE, further purified SP was obtained. The Mr of SP is 20.1 kDa. Antisera against the crude and further purified SP were prepared with the titer of 51 200 and 6 400, respectively. The antiserum against the further purified SP was proved to be highly specific, with which the serological relationships between RSV SP and RSV CP, CP and SP of rice grassy stunt virus (RGSV), another member of \%Tenuivirus\% were studied. Using PAS ELISA, it was found that RSV SP antiserum did not react with RSV CP and RGSV CP, but weakly reacted with RGSV SP. No serological relationship existed between RSV SP and the antisera raised against RSV CP, RGSV SP and RGSV CP, respecti