The Assem bly and Release of Sim ian AIDS Type D Retrovirus （SRV)：Involvement of Intermediate Filament

WEN Jian-Fan; HUANG Sheng-Min; ZHENG Yong-Tang

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June 1999

WEN Jian-Fan, HUANG Sheng-Min and ZHENG Yong-Tang. The Assem bly and Release of Sim ian AIDS Type D Retrovirus （SRV)：Involvement of Intermediate Filament[J]. Virologica Sinica, 1999, 14(3): 231-235.

Citation: WEN Jian-Fan, HUANG Sheng-Min, ZHENG Yong-Tang. The Assem bly and Release of Sim ian AIDS Type D Retrovirus （SRV)：Involvement of Intermediate Filament .VIROLOGICA SINICA, 1999, 14(3) : 231-235.

猴艾滋病病毒(SRV)的装配及释放与细胞内骨架系统关系的电镜观察

1.
中国科学院昆明动物研究所细胞与分子进化开放实验室，昆明，650223

摘要

将猴艾滋病Ｄ型逆转录病毒（ＳｉｍｉａｎＡＩＤＳｔｙｐｅＤｒｅｔｒｏｖｉｒｕｓ，ＳＲＶ１）感染的Ｒａｊｉ细胞进行选择性抽提制备成核骨架中间纤维这一细胞内骨架系统，再结合常规电镜技术和ＤＧＤ包埋去包埋剂电镜技术，对此病毒在宿主细胞内的装配和释放与这一骨架系统的关系进行了研究。结果表明ＳＲＶ１病毒核壳体的装配是在细胞核附近的胞质中进行的，其装配中心是结合在胞质骨架的中间纤维上，正在装配的和已装配好的病毒核壳体都是紧密结合在中间纤维上的。而在核内骨架系统中未观察到病毒粒子。这表明ＳＲＶ的装配可能是依赖于胞质中的中间纤维作支架，且装配好的核壳体可能沿着中间纤维被运送至质膜内侧或胞质内病毒感染后形成的空泡膜上，然后出芽释放
- 猴艾滋病D型逆转录病毒（SRV-1）
- , 病毒装配
- , 中间纤维

The Assem bly and Release of Sim ian AIDS Type D Retrovirus （SRV)：Involvement of Intermediate Filament

1.
Kunming Institute of Zoology，Chinese Academy of Sciences, Kunming 650223

Abstract

By conventional EM technique and DGD embedment free section EM technique together with the procedure of gentle extraction, the assembly and release of simian AIDS type D retrovirus (SRV) in infected Raji cells were studied. The results showed that SRV nucleocapsids were assemblied in some cytoplasm areas near nuclei, and these areas or virus assembly centers were suspended in the intermediate filament network; and the assembling and assembled virus nucleocapsids were associated firmly with intermediate filaments. It is suggested that the assembly of SRV nucleocapsids depends on intermediate filaments as support, and the assembled nucleocapsids may move along the intermediated filaments to cytoplasm membrane where they bud and are released.
- Simian AIDS type D retrovirus （SRV 1）
- , Virus assembly
- , Intermediate filament

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Assem bly and Release of Sim ian AIDS Type D Retrovirus （SRV)：Involvement of Intermediate Filament

1. Kunming Institute of Zoology，Chinese Academy of Sciences, Kunming 650223

Abstract: By conventional EM technique and DGD embedment free section EM technique together with the procedure of gentle extraction, the assembly and release of simian AIDS type D retrovirus (SRV) in infected Raji cells were studied. The results showed that SRV nucleocapsids were assemblied in some cytoplasm areas near nuclei, and these areas or virus assembly centers were suspended in the intermediate filament network; and the assembling and assembled virus nucleocapsids were associated firmly with intermediate filaments. It is suggested that the assembly of SRV nucleocapsids depends on intermediate filaments as support, and the assembled nucleocapsids may move along the intermediated filaments to cytoplasm membrane where they bud and are released.