Com puter-Based Prediction and Experim ental Confirm ation of the jSR Membrane Protein of African Swine Fever Viru

SUN Fu-Chang; LindaK.Dixon; R.M.EParkhouse

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June 1999

SUN Fu-Chang, LindaK.Dixon and R.M.EParkhouse. Com puter-Based Prediction and Experim ental Confirm ation of the jSR Membrane Protein of African Swine Fever Viru[J]. Virologica Sinica, 1999, 14(3): 236-243.

Citation: SUN Fu-Chang, LindaK.Dixon, R.M.EParkhouse. Com puter-Based Prediction and Experim ental Confirm ation of the jSR Membrane Protein of African Swine Fever Viru .VIROLOGICA SINICA, 1999, 14(3) : 236-243.

非洲猪瘟病毒j5R膜蛋白的电脑预测和实验证实

1.
扬州大学畜牧兽医学院动医系扬州225009
2.
InstituteforAnimalHealth AshRoad Pirbright SurreyGU24ONF UK

摘要

蛋白多肽二级结构的电脑预测表明，非洲猪瘟病毒（Ａｆｒｉｃａｎｓｗｉｎｅｆｅｖｅｒｖｉｒｕｓ，ＡＳＦＶ）ｊ５Ｒ阅读框编码１２．９ｋＤａ膜蛋白。该蛋白的Ｃ末端含有一个潜在抗原决定簇，针对其合成肽的抗体能在ＡＳＦＶ感染细胞和病毒颗粒中检测到２３或２５ｋＤａ（取决于不同毒株）特异蛋白。免疫荧光试验显示，ｊ５Ｒ蛋白主要位于感染细胞的病毒复制部位。油水两相分离和细胞分级分离试验结果证明ｊ５Ｒ蛋白是膜相关蛋白
- 非洲猪瘟病毒
- , j5R阅读框
- , 膜蛋白

Com puter-Based Prediction and Experim ental Confirm ation of the jSR Membrane Protein of African Swine Fever Viru

1.
Yangzhou University, Department of Animal Husbandry and Veterinary Medicine College of moving,Yangzhou 225009
2.
InstituteforAnimalHealth AshRoad Pirbright SurreyGU24ONF UK

Abstract

The j5R open reading frame (ORF) of African swine fever virus was predicted by computing to encode a 12.9 kDa protein with two successive N terminal transmembrane domains and one C terminal antigenic epitope. Antibodies raised against a synthetic peptide derived from the C terminal epitope detected a specific protein of 23 or 25 kDa (depending on different isolates) in ASFV infected cells or purified extracellular ASFV virions. Immunofluorescence showed that the j5R protein was mainly located in the virus assembly sites within ASFV infected cells. Phase separation of purified extracellular ASFV virions and fractionation of ASFV infected cells demonstrated that the j5R protein was in the detergent phase and membrane fraction, confirming that the j5R protein was membrane associated.
- African swine fever virus（ASFV）
- , j5R ORF
- , Membrane protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Com puter-Based Prediction and Experim ental Confirm ation of the jSR Membrane Protein of African Swine Fever Viru

1. Yangzhou University, Department of Animal Husbandry and Veterinary Medicine College of moving,Yangzhou 225009
2. InstituteforAnimalHealth AshRoad Pirbright SurreyGU24ONF UK

Abstract: The j5R open reading frame (ORF) of African swine fever virus was predicted by computing to encode a 12.9 kDa protein with two successive N terminal transmembrane domains and one C terminal antigenic epitope. Antibodies raised against a synthetic peptide derived from the C terminal epitope detected a specific protein of 23 or 25 kDa (depending on different isolates) in ASFV infected cells or purified extracellular ASFV virions. Immunofluorescence showed that the j5R protein was mainly located in the virus assembly sites within ASFV infected cells. Phase separation of purified extracellular ASFV virions and fractionation of ASFV infected cells demonstrated that the j5R protein was in the detergent phase and membrane fraction, confirming that the j5R protein was membrane associated.