JIA Wei, FENG Feng, LI Tian-Xian, CHEN Min-Liang and DIAO Lin. Identification and BiochemicaI Characteristics of a NeW Psittacine Virus in China[J]. Virologica Sinica, 1999, 14(3): 265-272.
Citation: JIA Wei, FENG Feng, LI Tian-Xian, CHEN Min-Liang, DIAO Lin. Identification and BiochemicaI Characteristics of a NeW Psittacine Virus in China .VIROLOGICA SINICA, 1999, 14(3) : 265-272.

我国一株鹦鹉病毒的鉴定及其生化特性

  • 1995年作者从湖北濒死鹦鹉体内分离到一株新病毒,经鹦鹉胚成纤维细胞盲传四代过渡到鸡胚成纤维细胞,再传至第五代出现细胞病变。适应细胞的病毒纯化后,经SDSPAGE分析,发现病毒粒子结构蛋白由8条多肽组成,分子量范围在14.5~60kD之间。病毒的酸碱氨基酸之比为2.51,富含Asp、Clu和Leu,而Arg和Met含量较少。病毒核酸为双链DNA,存在超螺旋型、环型和线型三种形态,G+C的含量为45%。经限制性内切酶分析,核酸的分子量为3.3106D。根据病毒分类的标准确认为澳州长尾小鹦鹉幼雏病病毒,应归属于乳多空病毒科多瘤病毒属。

Identification and BiochemicaI Characteristics of a NeW Psittacine Virus in China

  • A strain of new virus was isolated from fledgling budgerigars by authors in 1995 Cytopathic effect became visible after the isolate was passaged 4 generations on budgerigar embryo fibroblast and 5 generations on Chicken embryo fibroblast.The SDS-PAGE proved that viral capsidxgas composed of eight polypeptides with molecular w |gbt from 60 000 tO 14 500 D.The protein WBtS rich in Asp,Glu and Leu,but comparatively poorer in Arg and M et.The ratio of acidicamino acid to basic amino acid WBS l:2.5 l T nudeic acid Was dsDNA and exi~ed as supercoiled circular.relaxed ciseulatand Iinear molecules G 十C of viral DNA was 45% The molecu 1ar weight oE DNA WBtS 3.3 106D restriction endonuclease analysis According to standards of virus classification.it is proved that the isolate was budgerigar fledgling disease virus which had placed into the Polyomavirus genus of Papovaviridae.

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    Identification and BiochemicaI Characteristics of a NeW Psittacine Virus in China

    • 1. Wuhan Institute of Virdogv,Academia Sinica.Wuhan 430071
    • 2. College of Life  

    Abstract: A strain of new virus was isolated from fledgling budgerigars by authors in 1995 Cytopathic effect became visible after the isolate was passaged 4 generations on budgerigar embryo fibroblast and 5 generations on Chicken embryo fibroblast.The SDS-PAGE proved that viral capsidxgas composed of eight polypeptides with molecular w |gbt from 60 000 tO 14 500 D.The protein WBtS rich in Asp,Glu and Leu,but comparatively poorer in Arg and M et.The ratio of acidicamino acid to basic amino acid WBS l:2.5 l T nudeic acid Was dsDNA and exi~ed as supercoiled circular.relaxed ciseulatand Iinear molecules G 十C of viral DNA was 45% The molecu 1ar weight oE DNA WBtS 3.3 106D restriction endonuclease analysis According to standards of virus classification.it is proved that the isolate was budgerigar fledgling disease virus which had placed into the Polyomavirus genus of Papovaviridae.

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