EB Virus Infection and M utation of p53 Suppressor Gene in Gastric M ucom a Dam ages

DIAO Jun-Li; MEI Ke-Yong; LONG Yan-Hua; FANG Xiang-Meng-

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August 1999

DIAO Jun-Li, MEI Ke-Yong, LONG Yan-Hua and FANG Xiang-Meng-. EB Virus Infection and M utation of p53 Suppressor Gene in Gastric M ucom a Dam ages[J]. Virologica Sinica, 1999, 14(4): 304-309.

Citation: DIAO Jun-Li, MEI Ke-Yong, LONG Yan-Hua, FANG Xiang-Meng-. EB Virus Infection and M utation of p53 Suppressor Gene in Gastric M ucom a Dam ages .VIROLOGICA SINICA, 1999, 14(4) : 304-309.

胃粘膜损伤中EB病毒感染与P53抑癌基因突变的研究

1.
中国科学院武汉病毒所，武汉430071
2.
武汉第三医院．武汉43006

摘要

为了探讨EB病毒（EBV）感染与胃癌发生的关系，应用PCR技术对166例胃粘膜损伤标本的EBVDNA进行检测．其中66例应用银染PCR－SSCP分析技术检测了p53exon5～8突变情况，10例应用直接法原位PCR技术检测了EBV在组织中的感染情况。结果166例标本中EBV感染率为30．1％；EBV在细胞中感染大体是弥散型，主要存在于细胞核。66例标本中p53基因突变率为54．5％。对比分析，EBV阳性标本中p53基因突变率为75％（21／28），EBV阴性标本中p53基因突变率为39．5％（15／38）。结果表明，EBV对胃粘膜组织细胞具有易感性，p53基因突变在胃粘膜病变中是一个常发事件，EBV感染与p53基因突变之间存在着高度相关性．对癌症的发生具有重要作用。
- EB病毒
- , p53基因
- , 聚合酶链反应
- , PCR-SSCP分析
- , 胃粘膜损伤

EB Virus Infection and M utation of p53 Suppressor Gene in Gastric M ucom a Dam ages

1.
Wuhan Institute of Virology，Academia Sinica，Wuhan 430071
2.
The ThirdHospitalof Wuhan，Wuhan 430060

Abstract

To investigate the relationship between EBV infection and genesis of gastric cancer,PCR, PCR-SSCP assay and direct in situ PCR technique were performed on samples of gastricmucoma damages; EBV infection rate was 30. 1 % in 166 samples; EBV was scattered in tissuesand roughly in nucleuses. p53 gene mutation rate was 54. 5% in 66 samples. In contrast, p53gene mutation rate was 75% in EBV-positive samples (21/28), but 39. 5 % in EBV-negative ones(15/38). The results suggest that EBV is infectious for gastric mucoma cells, p53 gene mutationis common in gastric mucoma pathological changes, EBV infection has high correlation with p53mutation, and is of importance during the genesis of cancer
- EB virus
- , p53 gene
- , Polymerese chain reaction
- , PCR-SSCP assay
- , Gastric muooma damage

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

EB Virus Infection and M utation of p53 Suppressor Gene in Gastric M ucom a Dam ages

1. Wuhan Institute of Virology，Academia Sinica，Wuhan 430071
2. The ThirdHospitalof Wuhan，Wuhan 430060

Abstract: To investigate the relationship between EBV infection and genesis of gastric cancer,PCR, PCR-SSCP assay and direct in situ PCR technique were performed on samples of gastricmucoma damages; EBV infection rate was 30. 1 % in 166 samples; EBV was scattered in tissuesand roughly in nucleuses. p53 gene mutation rate was 54. 5% in 66 samples. In contrast, p53gene mutation rate was 75% in EBV-positive samples (21/28), but 39. 5 % in EBV-negative ones(15/38). The results suggest that EBV is infectious for gastric mucoma cells, p53 gene mutationis common in gastric mucoma pathological changes, EBV infection has high correlation with p53mutation, and is of importance during the genesis of cancer