JI Wei, WANG Qi-Huan, SHI Chong-Wen, XU Min, ZHANG Guo-Qiang and LIU Dan. Inhibition of Hepatitis B Virus by Retroviral Vectors Expressing Antisenses PreS/S[J]. Virologica Sinica, 1999, 14(4): 310-313.
Citation: JI Wei, WANG Qi-Huan, SHI Chong-Wen, XU Min, ZHANG Guo-Qiang, LIU Dan. Inhibition of Hepatitis B Virus by Retroviral Vectors Expressing Antisenses PreS/S .VIROLOGICA SINICA, 1999, 14(4) : 310-313.

反义前S/S基因转移表达抗乙型肝炎病毒的研究

  • 为了观察反义基因转录表达抗乙型肝炎病毒(HBV)的作用,将HBVayw前S/S基因(PreS/S)片段反向插入逆转录病毒载体质粒,构建preS/S反义基因重组体,经转染PA317细胞后,获得重组逆转录病毒颗粒。用重组病毒转导2.2.15细胞后,第3天即可见细胞培养上清HBV表面抗原(HBsAg)和e抗原(HBeAg)表达量减少,到转导后第5天,细胞培养上清中的HBsAg和HBeAg表达量降到最低水平,HBsAg抑制率为71%,HBeAg抑制率为27%,抑制作用至少可持续到转导后第11天。空载及正向插入基因的重组逆转录病毒转导对2,2.15细胞内HBV抗原表达无抑制作用。

Inhibition of Hepatitis B Virus by Retroviral Vectors Expressing Antisenses PreS/S

  • Retroviral vector expressing antisense RNA complementary to HBV preS/S (antisenseS) was used to transduce human hepatoblastoma cell 2. 2. 15, which was trans fected with HBVDNA and can express HBV markers. The results showed that the inhibitory effects of antisensegene transfer mediated by retroviral vector antisense-S on the expression of HBV antigens appeared as early as on day 3 after transduction, reached peak level on day 5, and persisted at leastfor 11 days. The inhibitory rates of HBsAg and HBeAg in the 2. 2. 15 cells by antisense-S were71% and 27% on day 5 after transduction, and in comparison to blank or sense-S, the inhibitoryeffects of antisense-S were highly significant (P0.01 ). The viability of transduced 2. 2. 15 cellswas not affected as indicated by MTT assays. The results demonstrated that retroviral vectorscontaining antisense gene can inhibit HBV, and the antisense retroviral vectors may be potentiallyuseful for anti-HBV gene therapy.

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      沈阳化工大学材料科学与工程学院 沈阳 110142

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    Inhibition of Hepatitis B Virus by Retroviral Vectors Expressing Antisenses PreS/S

    • 1. The Seventh Department ofInfectious Diseases,302 Hospital of the PLA,Beijing 100039
    • 2. Department of Infectious Diseases,The First Affiliated Hospital,Bdjing Medical University,Beijing 100034

    Abstract: Retroviral vector expressing antisense RNA complementary to HBV preS/S (antisenseS) was used to transduce human hepatoblastoma cell 2. 2. 15, which was trans fected with HBVDNA and can express HBV markers. The results showed that the inhibitory effects of antisensegene transfer mediated by retroviral vector antisense-S on the expression of HBV antigens appeared as early as on day 3 after transduction, reached peak level on day 5, and persisted at leastfor 11 days. The inhibitory rates of HBsAg and HBeAg in the 2. 2. 15 cells by antisense-S were71% and 27% on day 5 after transduction, and in comparison to blank or sense-S, the inhibitoryeffects of antisense-S were highly significant (P0.01 ). The viability of transduced 2. 2. 15 cellswas not affected as indicated by MTT assays. The results demonstrated that retroviral vectorscontaining antisense gene can inhibit HBV, and the antisense retroviral vectors may be potentiallyuseful for anti-HBV gene therapy.

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