根据HFRSV汉滩型（HTN）代表株76－118和汉城型（SEO）代表株R22基因资料，设计了两组引物，用电脑软件分析证明设计符合引物标准。以一组引物克隆全长S基因片段和N端的部分S基因片段，并使它们在T7系统进行融合表达和非融合表达。非融合表达产量虽不及融合表达高，但生物活性好。以非融合表达的两个S基因片段产物作间接ELISA的包被抗原，其工作浓度均达1：100000用另一组引物建立了逆转录-聚合酶链式反应（RT-PCR），检测我国不同地区由8种主要宿主分离的37个HFRSV毒株，2个阳性标准对照毒株和5个阴性对照标本，并与cELISA法比较，二者阳性检出率分别为100％和84．6％，符合率为84．6％，但前者比后者敏感性高15．4％。对其中20个毒株的PCR扩增产物先后用RsaⅠ和HindⅢ作二级酶切，建立了逆转录-聚合酶链式反应-限制性片段长度多态性分析（RT-PCR－RFLP）分型法。被定为HTN型的9株，SEO型的8株，余3株未能定型。此20个毒株曾用血清学方法分型，仅11株分型成功．与RT-PCR－RFLP法结果符合。分型成功率RT-PCR－RFLP法比血清法高30％。

Two groups of primers were designed according to the gene backgrounds of prototype virus of HTN and SEO serotypes and corrected by co mputer．One group of primers was used to elone entire S gencane segment and partial S genome segment with respect to N．terrmnal，n 1 two cloned genes were fusionally expressed and non-fusionally expressed by 27 system ．The non fusionally expressed products whose working co ncentration were 1：10 000 presented a good bi0_ logical activity though their yields were lower than the fusionally expressed products．The other group of primers was used to establish a meth0d of RTPCR to detect RNAs of 37 virus isolates of HFRSv 2 positeve standard viruses and 5 negtive controls． On comparision with that of cELISA，the detecting rates of two m ethods were 100％ and 84．6％ respectively，the co incidental ratewas 84．6％ whilethefoiT~1er had 15．4％ higher sensitivitythan thelatter．Thetyping method of RFLP was set up by digesting the PCR products of 20 virus isolates with Ras I and HindUl resulting that 9 OUt of the tot81 were HTN．8 were SEO aM 3 were n0t determined re spectively．The same 20 viruses have been previoushy typed using serotyping method resulting tha t only 11 could he typed successfully．showing a high co nsistency with that of RTPCR-RFLP method and a 30％ lower typing etficiency than the latter.