LIN Han-Xin, LIN Ai-Tian, TUN Jie-Jian, LIN Ai-Yang and XIE Lian-Hui. Characterization of Proteins and Nucleic Acid of Rice Stripe Virus[J]. Virologica Sinica, 1999, 14(4): 343-352.
Citation: LIN Han-Xin, LIN Ai-Tian, TUN Jie-Jian, LIN Ai-Yang, XIE Lian-Hui. Characterization of Proteins and Nucleic Acid of Rice Stripe Virus .VIROLOGICA SINICA, 1999, 14(4) : 343-352.

水稻条纹病毒蛋白与核酸的特性

  • 提纯的水稻条纹病毒(云南宜良分离物)在电镜下的形态为多型性,但主要是宽8-10urn,长80-25O的分枝丝状体,有些为直径3urn或8urn的开环环状体,有些为13urn宽,130-190urn长的丝状体,但其基本结构应是直径3urn、长度不等的丝状体。经聚丙烯酸胺凝胶电泳分析,VRNA4编码的病害特异蛋白(SP)分子量为19.9kDa,而VCRNA3编码的外壳蛋白(CP)约为336kDa。在非变性条件下,RSV的4条ssRNAs大小分别为3OXIO(ssRNAI)、互.2XIc(ssR.NAZ)、0.9X10(ssRNA3)和0.8X10Da(SSRNA4),有时出现一条大小为0.58X10Da的单链RNA(ssRNA);而4条dsRNAs的分子量分别为4.9X10(dsRNAI)、2.8X10(dsRNAZ)、20XIOo(dsRNA3)和1.7X10D。(dSRNA4)。利用制备电泳分离提纯的外壳蛋白免疫家兔,得到了高特异性的抗血清。A蛋白夹心ELISA检测结果表服RSV-CP与水稻草状矮化病毒(RGSV)CP抗血清有微弱的反应,但与RSV、RGSV的SP抗血清没有反应,而RSV-CP抗血清与RSV.SP及RGSV的SP、CP都无血清学关系,这个结果表明RGSV与RSV之间在进化上具有一定的亲缘关系。

Characterization of Proteins and Nucleic Acid of Rice Stripe Virus

  • An isolate of rice stripe virus (designated as RSVYL) was purified. The particlesshowed to be pleomorphisms under electron microscope, mainly branched filamnts of about 80-250 nm in length and abeut 8 nm in width. There are also some open circular filaments of 3nm and 8 nm in width, and some filaments of 13 nm in width and 130-190 nm in length.The basic morphism of RSV particles should be filaments of 3 nm in width and various length.By SDS-PAGE analysis, the molecular weight of disease-specific protein (SP) encoded by vRNA4 was 19. 9 kDa and tha of coat protein (CP) encoded by vcRNA3 was 33. 6 kDa. Whennucleic acid extrcted from the purified RSV was electrophoresed under nondenaturing condition, the she of four dsRNAs(designated as dsRNA1-4 in order of decreasing size) was 4. 9106, 2. 7106, 2.0106 and 1. 7106 Da, respectively, and that of four ssRNAs (designated as ssRNA1-4 in order of decreasing size) was 3. 0106, 1. 2106, 0. 9 106 and 0. 8106 De, respectively. A fifth Segment with a size of 0. 58106

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    Characterization of Proteins and Nucleic Acid of Rice Stripe Virus

    • 1. Institute of Plant Virology of Fujian Agricultural University,Key Laboratory of Plant Virology of Fujian Province,Fuzhou 350002

    Abstract: An isolate of rice stripe virus (designated as RSVYL) was purified. The particlesshowed to be pleomorphisms under electron microscope, mainly branched filamnts of about 80-250 nm in length and abeut 8 nm in width. There are also some open circular filaments of 3nm and 8 nm in width, and some filaments of 13 nm in width and 130-190 nm in length.The basic morphism of RSV particles should be filaments of 3 nm in width and various length.By SDS-PAGE analysis, the molecular weight of disease-specific protein (SP) encoded by vRNA4 was 19. 9 kDa and tha of coat protein (CP) encoded by vcRNA3 was 33. 6 kDa. Whennucleic acid extrcted from the purified RSV was electrophoresed under nondenaturing condition, the she of four dsRNAs(designated as dsRNA1-4 in order of decreasing size) was 4. 9106, 2. 7106, 2.0106 and 1. 7106 Da, respectively, and that of four ssRNAs (designated as ssRNA1-4 in order of decreasing size) was 3. 0106, 1. 2106, 0. 9 106 and 0. 8106 De, respectively. A fifth Segment with a size of 0. 58106

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