Identification and primary application of fusion-inhibiting active monoclonal antibody of respira- tory syncytial virus

HUANG Sheng-Hai; SHI Bai-Fen; WANG Meng-Li; ZHANG Yong-Na; HU Yong; BI Ke-Ju

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February 2000

HUANG Sheng-Hai, SHI Bai-Fen, WANG Meng-Li, ZHANG Yong-Na, HU Yong and BI Ke-Ju. Identification and primary application of fusion-inhibiting active monoclonal antibody of respira- tory syncytial virus[J]. Virologica Sinica, 2000, 15(1).

Citation: HUANG Sheng-Hai, SHI Bai-Fen, WANG Meng-Li, ZHANG Yong-Na, HU Yong, BI Ke-Ju. Identification and primary application of fusion-inhibiting active monoclonal antibody of respira- tory syncytial virus .VIROLOGICA SINICA, 2000, 15(1) : 34.

呼吸道合胞病毒融合抑制活性单克隆抗体的鉴定及应用

1.
安徽医科大学微生物学教研室

摘要

用杂交瘤技术制备了抗呼吸道合胞病毒 (RSV Long株 )的单克隆抗体F3细胞株。经IFA及ELISA证明 ,F3株McAb对RSV抗原特异 ,具中和活性及融合抑制活性 ,其腹水中和效价和融合抑制效价分别为 1∶12 8和 1∶6 4。将F3株McAb与商售混合单抗试剂盒进行临床诊断比较 ,二者阳性符合率 96 .9% ,阴性符合率 100 %。
- 呼吸道合胞病毒（RSV）单克隆抗体（McAb）融合抑制试验诊断

Identification and primary application of fusion-inhibiting active monoclonal antibody of respira- tory syncytial virus

1.
Anhui Medical University

Abstract

Monoclonal antibody of F 3 hybridoma cell strain against RSV specific antigen was prepared by hybridoma and tested by indirect immunofluorescence and ELISA. It has neutralizing and fusion inhibiting activity. The neutralizing titres and fusion inhibiting titres of ascites of mice are 1∶128 and 1∶64 respectively. Clinical diagnosis by comparing McAb of F 3 strain with fixed McAb of commerical kit show that the positive according rate are 96.9% and negative according rate are 100%. F 3 hybridoma cell strain probably produces a monoclonal antibody against fusion protein of RSV.
- Respiratory syncytial virus Monoclonal antibody Fusion inhibiting test Clinical diagnosis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Identification and primary application of fusion-inhibiting active monoclonal antibody of respira- tory syncytial virus

1. Anhui Medical University

Abstract: Monoclonal antibody of F 3 hybridoma cell strain against RSV specific antigen was prepared by hybridoma and tested by indirect immunofluorescence and ELISA. It has neutralizing and fusion inhibiting activity. The neutralizing titres and fusion inhibiting titres of ascites of mice are 1∶128 and 1∶64 respectively. Clinical diagnosis by comparing McAb of F 3 strain with fixed McAb of commerical kit show that the positive according rate are 96.9% and negative according rate are 100%. F 3 hybridoma cell strain probably produces a monoclonal antibody against fusion protein of RSV.