LI Qing-Hong, HUANG Ru-Tong, WEI Zhao-Jing and LI De-Rong. Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou[J]. Virologica Sinica, 2000, 15(1).
Citation: LI Qing-Hong, HUANG Ru-Tong, WEI Zhao-Jing, LI De-Rong. Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou .VIROLOGICA SINICA, 2000, 15(1) : 39.

广州地区急性散发性HEV毒株基因特征和生物学性状的研究

  • 利用RT PCR技术对我国广州地区急性散发性戊型肝炎病毒细胞培养分离株G93 1、G93 2、G93 3和G93 4基因组聚合酶区部分核苷酸序列 (4 52 2~ 4 76 1nt)进行检测 ,PCR阳性产物经纯化、克隆后测序。结果G93 1、G93 3和G93 4株病毒的这段序列完全相同 ,且与我国新疆暴发流行的HEV 87A株及亚洲HEV代表株的同源性为 10 0 % ;而G93 2株与它们的差异较大 ,同源性只有 79.9% ;但是 ,它与 1997年从厦门地区急性戊型肝炎病人血清中检测的X S1株同源性很高 ,达99.2 %。并对G93 2株病毒的生物学特性进行了研究 ,结果与 87A株一致。表明我国南方散发性HEV毒株的基因组存在差异 ,可能同时流行着两种不同的HEV基因型 ,但生物学性状是相同的。

Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou

  • Partial nucleotide sequences (location 4522-4761) from ORF1 of sporadic strains (G93 1,G93 2,G93 3 and G93 4) of acute HEV in Guangzhou were detected, the positive PCR products were purified, cloned and sequenced. In the result, the nucleotide sequences of G93 1, G93 3 and G93 4 strain are all the same to each other, and have a homology of 100% with the Xin Jiang 87A and Burma strain respectively. The G93 2 strain is different, the homology of this part of sequence with them is only 79.9%, but it has a homology of 99.2% with X S1 strain obtained from the acute phase serum of a patient with hepatitis E in Xiamen. In addition, the biological character of the G93 2 strain was studied, the result was as same as the 87A strain's. All the results show there is a variant subgenotype of HEV in the South of China besides the similar strain with Xinjiang, but they have the same biological character.

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    Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou

    • 1. Military Institute of Medical Science

    Abstract: Partial nucleotide sequences (location 4522-4761) from ORF1 of sporadic strains (G93 1,G93 2,G93 3 and G93 4) of acute HEV in Guangzhou were detected, the positive PCR products were purified, cloned and sequenced. In the result, the nucleotide sequences of G93 1, G93 3 and G93 4 strain are all the same to each other, and have a homology of 100% with the Xin Jiang 87A and Burma strain respectively. The G93 2 strain is different, the homology of this part of sequence with them is only 79.9%, but it has a homology of 99.2% with X S1 strain obtained from the acute phase serum of a patient with hepatitis E in Xiamen. In addition, the biological character of the G93 2 strain was studied, the result was as same as the 87A strain's. All the results show there is a variant subgenotype of HEV in the South of China besides the similar strain with Xinjiang, but they have the same biological character.

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